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Anti-RanBP1 antibody (ab17034)

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Overview

Product name

Anti-RanBP1 antibody
See all RanBP1 products (6) ...

Description

Goat polyclonal to RanBP1

Tested applications

WB, ELISAmore details

Cross reactivity

Reacts with

Human

Immunogen

Synthetic peptide: LSVKEETKEDAEEKQ, corresponding to amino acids 187-201 of Human RanBP1.

C-LSVKEETK EDAEEKQ

Run BLAST with BLAST the sequence with ExPASyRun BLAST with BLAST the sequence with NCBI

Positive control

HeLa and HepG2 whole cell lysate.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline. pH 7.3

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Clonality

Polyclonal

Isotype

IgG

Research areas

Epigenetics and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Other
Signal Transduction >> Protein Trafficking >> Nuclear Import / Export
Cell Biology >> Cell Cycle >> Cell Division >> Spindle

  • Western blot - RanBP1 antibody (ab17034)Western blot - RanBP1 antibody (ab17034) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab17034 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide1 Image

    WB

     WB: Use a concentration ...Read more →

    WB: Use a concentration of 0.1 - 1 µg/ml.Detects a band of approximately 28-30 kDa (predicted molecular weight: 23 kDa).

  • ELISA

     ELISA: 1/16000

    ELISA: 1/16000

Target

Function

Inhibits GTP exchange on Ran. Forms a Ran-GTP-RANBP1 trimeric complex. Increase GTP hydrolysis induced by the Ran GTPase activating protein RANGAP1. May act in an intracellular signaling pathway which may control the progression through the cell cycle by regulating the transport of protein and nucleic acids across the nuclear membrane.

Sequence similarities

Belongs to the RANBP1 family.
Contains 1 RanBD1 domain.

Target information above from: UniProt accessionP43487 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • HTF9A antibody
  • RAN binding protein 1 antibody
  • Ran specific GTPase activating protein antibody
  • Ran-binding protein 1 antibody
  • Ran-specific GTPase-activating protein antibody
  • RANBP1 antibody
  • RANG_HUMAN antibody
see all

Database links

Anti-RanBP1 antibody images:

  Western blot - RanBP1 antibody (ab17034)

Western blot - RanBP1 antibody (ab17034)



Predicted band size : 23 kDa


ab17034 at 0.1µg/ml staining RanBP1 from HepG2 lysate (35µg total protein per lane) by Western blot. The primary antibody was incubated for 1 hour. The blot was detected by chemiluminescence.

References for Anti-RanBP1 antibody (ab17034)

ab17034 has not yet been referenced specifically in any publications.

Publishing research using ab17034? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"