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Western blot - RanBP1 antibody (ab17034)
Western blot - RanBP1 antibody (ab17034)
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Product Name 

RanBP1 antibody

 

See all RanBP1 antibodies (3)...

Product type 

Primary antibodies

Description 

Goat polyclonal to RanBP1

Immunogen 

Synthetic peptide: LSVKEETKEDAEEKQ, corresponding to amino acids 187-201 of Human RanBP1.
BLAST 'C-LSVKEETKEDAEEKQ' with BLAST the sequence with ExPASy or BLAST the sequence with NCBI

Reacts with 
(species key)

Hu

Tested applications 
(see key)

ELISA, WB


Abreviews 

Customer reviews
(see key)


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Application notes 
(see key)

Recommended dilutions

ELISA: 1/16000.
WB: Use at a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 28-30 kDa (predicted molecular weight: 23 kDa).

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

HeLa and HepG2 whole cell lysate.

Research areas 

Chromatin and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Other
Signal Transduction >> Protein Trafficking >> Nuclear Import / Export
Cell Biology >> Cell Cycle >> Cell Division >> Spindle

Relevance 

Transport of macromolecules into and out of the nucleus occurs through nuclear pore complexes (NPC). This energy-dependent process involves nucleoporins at the NPC and associated transport proteins and GTP. Ran binding protein 1 (RanBP 1) belongs to a family of proteins that bind Ran GTPase which is essential for mRNA processing, nuclear transport, cell cycle control, mitotic spindle assembly, and postmitotic nuclear re-assembly and nuclear architecture maintenance.

 

Alternative names RanBP1 antibody (ab17034)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

5902    (Human)

Omim

    

601180    (Human)

SwissProt

    

P43487    (Human)

Unigene

    

24763    (Human)

Raised in 

Goat

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline. pH 7.3

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

0.500 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this RanBP1 antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for RanBP1 antibody images, references, products related to ab17034 and other tools.

 

RanBP1 antibody images:

Product image  Western blot - RanBP1 antibody (ab17034)

Western blot - RanBP1 antibody (ab17034)



Predicted band size : 23 kDa


ab17034 at 0.1µg/ml staining RanBP1 from HepG2 lysate (35µg total protein per lane) by Western blot. The primary antibody was incubated for 1 hour. The blot was detected by chemiluminescence.

 
 

Search PubMed (MEDLINE) for references to RanBP1

 

RanBP1 antibody - more information

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Products for Western blot

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Lysates

 

Immunizing Peptide for RanBP1 antibody

ab23305

  

RanBP1 peptide (187-201)

 

Positive Controls for RanBP1 antibody

ab7900

  

HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate

 

Compatible Secondaries for RanBP1 antibody

ab6566

  

Cy5 ® antibody

ab6739

  

Texas Red ® antibody

ab6740

  

Biotin antibody

ab6741

  

HRP antibody

ab6742

  

Alkaline Phosphatase antibody

ab6881

  

FITC antibody

ab7004

  

Phycoerythrin antibody

ab27245

  

10nm Gold antibody

ab27246

  

10nm Gold antibody

ab27247

  

15nm Gold antibody

 

Other RanBP1 Antibodies

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