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If your product does not perform as described on this datasheet, we will refund or replace your product...
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Western blot - RanBP1 antibody (ab70185)
Lane 1 : Anti-RanBP1 antibody (ab70185) at 1 µg/ml
Lane 2 : Anti-RanBP1 antibody (ab70185) at 0.33 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : HeLa whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size : 23 kDa
Observed band size : 28 kDa (why is the actual band size different from the predicted?)
Exposure time : 5 minutes
Immunoprecipitation - RanBP1 antibody (ab70185)
Western Blot showing ab70185 at 1µg/ml staining RanBP1 in HeLa whole cell lysates immunoprecipitated using ab70185 at 5µg/0.5mg lysate (Lane 1). Lane 2: Control IgG was used for immunoprecipitation. Detection: Chemiluminescence with an exposure time of 5 minutes
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