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Products:Cell Biology >> Cell Cycle >> Cell Division >> Spindle
Overview
Product name
Anti-RanBP1 antibody
See all RanBP1 products (6) ...
Description
Rabbit polyclonal to RanBP1
Tested applications
WB, IPmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthetic peptide corresponding a region between residues 1 and 50 of human RanBP1 (NP_002873.1)
Positive control
HeLa whole cell lysates.
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
ab70185 was affinity purified using an epitope specific to RanBP1 immobilized on solid support.
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Other
Signal Transduction >> Protein Trafficking >> Nuclear Import / Export
Cell Biology >> Cell Cycle >> Cell Division >> Spindle
Applications
Show applications key
Our Abpromise guarantee covers the use of ab70185 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
IP: Use at 2-5µg/mg of lysate.
WB: 1/1000 - 1/5000. Detects a band of approximately 28 kDa (predicted molecular weight: 23 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Inhibits GTP exchange on Ran. Forms a Ran-GTP-RANBP1 trimeric complex. Increase GTP hydrolysis induced by the Ran GTPase activating protein RANGAP1. May act in an intracellular signaling pathway which may control the progression through the cell cycle by regulating the transport of protein and nucleic acids across the nuclear membrane.
Sequence similarities
Belongs to the RANBP1 family.
Contains 1 RanBD1 domain.
Target information above from: UniProt accessionP43487
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- HTF9A antibody
- RAN binding protein 1 antibody
- Ran specific GTPase activating protein antibody
see all
Database links
- Entrez Gene: 5902 Human
- Entrez Gene: 19385 Mouse
- Omim: 601180 Human
- SwissProt: P43487 Human
- SwissProt: P34022 Mouse
- Unigene: 24763 Human
- Unigene: 235287 Mouse
- Unigene: 3752 Mouse
Anti-RanBP1 antibody images:
Western blot - RanBP1 antibody (ab70185)
Lane 1 : Anti-RanBP1 antibody (ab70185) at 1 µg/ml
Lane 2 : Anti-RanBP1 antibody (ab70185) at 0.33 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : HeLa whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size : 23 kDa
Observed band size : 28 kDa (why is the actual band size different from the predicted?)
Exposure time : 5 minutes
Immunoprecipitation - RanBP1 antibody (ab70185)
Western Blot showing ab70185 at 1µg/ml staining RanBP1 in HeLa whole cell lysates immunoprecipitated using ab70185 at 5µg/0.5mg lysate (Lane 1). Lane 2: Control IgG was used for immunoprecipitation. Detection: Chemiluminescence with an exposure time of 5 minutes
References for Anti-RanBP1 antibody (ab70185)
ab70185 has not yet been referenced specifically in any publications.
Publishing research using ab70185? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"