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Products:Cell Biology >> Cell Cycle >> Cell Division >> Centromere
Overview
Product name
Anti-RanBP9 antibody
See all RanBP9 products (3) ...
Description
Goat polyclonal to RanBP9
Tested applications
IP, ELISA, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthetic peptide: GSCAFATVEDYLH, corresponding to C terminal amino acids 717-729 of Human RanBP9.
GSCAFATVED YLH
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris-saline. pH 7.3
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Cell cycle >> Chromosome Structure >> Centromere
Cell Biology >> Cell Cycle >> Cell Division >> Centromere
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - RanBP9 antibody (ab5295)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab5295 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP: Use at an assay dependent concentration. (PubMed: 21561988)
ELISA: Use at an assay dependent dilution. (Peptide ELISA: Antibody detection limit dilution 1:16,000)
IHC-P: Use a concentration of 3 µg/ml
Target
Function
May act as an adapter protein to couple membrane receptors to intracellular signaling pathways. May be involved in signaling of ITGB2/LFA-1 and other integrins. Enhances HGF-MET signaling by recruiting Sos and activating the Ras pathway. Involved in activation of androgen and glucocorticoid receptor in the presence of their cognate hormones. Stabilizes TP73 isoform Alpha, probably by inhibiting its ubiquitination, and increases its proapoptotic activity. Inhibits the kinase activity of DYRK1A and DYRK1B. Inhibits FMR1 binding to RNA.
Tissue specificity
Ubiquitously expressed, with highest levels in testes, placenta, heart, and muscle, and lowest levels in lung. Within the brain, expressed predominantly by neurons in the gray matter of cortex, the granular layer of cerebellum and the Purkinje cells.
Sequence similarities
Belongs to the RANBP9/10 family.
Contains 1 B30.2/SPRY domain.
Contains 1 CTLH domain.
Contains 1 LisH domain.
Domain
The SPRY domain mediates the interaction with MET, AR, and CDC2L1.
Post-translational
modifications
Phosphorylated upon DNA damage, probably by ATM or ATR. Phosphorylated in response to stress. Can be phosphorylated by the cleaved p110 form of CDC2L1 (p110C).
Ubiquitinated. Polyubiquitination targets the protein for rapid degradation via the ubiquitin system. Can be deubiquitinated by USP11.
Cellular localization
Nucleus. Cytoplasm. A phosphorylated form is associated with the plasma membrane.
Target information above from: UniProt accessionQ96S59
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- B cell antigen receptor Ig beta associated protein 1 antibody
- BPM 90 antibody
- BPM L antibody
see all
Database links
- Entrez Gene: 10048 Human
- Entrez Gene: 56705 Mouse
- Omim: 603854 Human
- SwissProt: Q96S59 Human
- SwissProt: P69566 Mouse
- Unigene: 708182 Human
- Unigene: 148781 Mouse
Anti-RanBP9 antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - RanBP9 antibody (ab5295)
Ab5295 (3ug/ml) staining of paraffin embedded Human Tonsil. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.
References for Anti-RanBP9 antibody (ab5295)
This product has been referenced in:
- Puverel Set al. RanBPM is essential for mouse spermatogenesis and oogenesis. Development 138:2511-21 (2011). IP; Mouse.Read more (PubMed: 21561988) »
- Atabakhsh Eet al. RanBPM has proapoptotic activities that regulate cell death pathways in response to DNA damage. Mol Cancer Res 7:1962-72 (2009).Read more (PubMed: 19996306) »
See all 3 publications for this product
Publishing research using ab5295? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"