RanBP9 antibody (ab5295)

Product Name 

RanBP9 antibody

Product type 

Primary antibodies

Description 

Goat polyclonal to RanBP9

Immunogen 

Synthetic peptide: GSCAFATVEDYLH, corresponding to C terminal amino acids 717-729 of Human RanBP9.
BLAST 'GSCAFATVEDYLH' with or

Reacts with 
(species key)

Hu, Ms

Tested applications 
(see key)

ELISA, IHC-P

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
Peptide ELISA: Antibody detection limit dilution 1:16,000.
IHC-P: Use at a concentration of 3 µg/ml. In paraffin embedded Human Tonsil, stains nuclei in epithelial cells of tonsillar criptae.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Cytoplasmic and Nuclear

Research areas 

Chromatin and Nuclear Signaling >> Cell cycle >> Chromosome Structure >> Centromere
Cell Biology >> Cell Cycle >> Cell Division >> Centromere

Relevance 

Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Mediates the nuclear import of H2B histone, RPS7 and RPL18A. Prevents the cytoplasmic aggregation of RPS7 and RPL18A by shielding exposed basic domains. May also import H2A, H3, H4 histones, RPL4 and RPL6.

Database links 

Raised in 

Goat

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris-saline. pH 7.3

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Purification notes 

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Form 

Liquid

Concentration 

0.500 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

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References for RanBP9 antibody (ab5295)

  This product has been used in: (two most recent references)

Atabakhsh E et al. RanBPM has proapoptotic activities that regulate cell death pathways in response to DNA damage. Mol Cancer Res 7:1962-72 (2009). PubMed: 19996306

Valiyaveettil M et al. Novel role of the muskelin-RanBP9 complex as a nucleocytoplasmic mediator of cell morphology regulation. J Cell Biol 182:727-39 (2008). WB; Mouse. PubMed: 18710924
If you publish research using ab5295 please let us know so that we can cite the reference on this datasheet.

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