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Products:Cell Biology >> Cell Cycle >> Cell Division >> Centromere
Overview
Product name
Anti-RanBP9 antibody
See all RanBP9 products (3) ...
Description
Rabbit polyclonal to RanBP9
Tested applications
WB, IP, ICC/IF, IHC-Pmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Chicken, Chimpanzee, Opossum
Immunogen
Recombinant fusion protein: PHGDSALNEQ EKELQRRLKR LYPAVDEQET PLPRSWSPKD KFSYIGLSQN NLRVHYKGHG KTPKDAASVR ATHPIPAACG IYYFEVKIVS KGRDGY, corresponding to amino acids 133-229 of Human RanBP9
PHGDSALNEQ EKELQRRLKR LYPAVDEQET PLPRSWSPKD KFSYIGLSQN NLRVHYKGHG KTPKDAASVR ATHPIPAACG IYYFEVKIVS KGRDGY
Positive control
Crude extracts of HeLa and U2OS cells.
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: Whole serum
Purity
Whole antiserum
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Cell cycle >> Chromosome Structure >> Centromere
Cell Biology >> Cell Cycle >> Cell Division >> Centromere
Applications
Show applications key
Our Abpromise guarantee covers the use of ab64275 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/2000Detects a band of approximately 95 kDa (predicted molecular weight: 78 kDa).
IP: Use at an assay dependent dilution.
ICC/IF: Use at an assay dependent dilution.
IHC-P: Use a concentration of 5 µg/mlPerform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Target
Function
May act as an adapter protein to couple membrane receptors to intracellular signaling pathways. May be involved in signaling of ITGB2/LFA-1 and other integrins. Enhances HGF-MET signaling by recruiting Sos and activating the Ras pathway. Involved in activation of androgen and glucocorticoid receptor in the presence of their cognate hormones. Stabilizes TP73 isoform Alpha, probably by inhibiting its ubiquitination, and increases its proapoptotic activity. Inhibits the kinase activity of DYRK1A and DYRK1B. Inhibits FMR1 binding to RNA.
Tissue specificity
Ubiquitously expressed, with highest levels in testes, placenta, heart, and muscle, and lowest levels in lung. Within the brain, expressed predominantly by neurons in the gray matter of cortex, the granular layer of cerebellum and the Purkinje cells.
Sequence similarities
Belongs to the RANBP9/10 family.
Contains 1 B30.2/SPRY domain.
Contains 1 CTLH domain.
Contains 1 LisH domain.
Domain
The SPRY domain mediates the interaction with MET, AR, and CDC2L1.
Post-translational
modifications
Phosphorylated upon DNA damage, probably by ATM or ATR. Phosphorylated in response to stress. Can be phosphorylated by the cleaved p110 form of CDC2L1 (p110C).
Ubiquitinated. Polyubiquitination targets the protein for rapid degradation via the ubiquitin system. Can be deubiquitinated by USP11.
Cellular localization
Nucleus. Cytoplasm. A phosphorylated form is associated with the plasma membrane.
Target information above from: UniProt accessionQ96S59
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- B cell antigen receptor Ig beta associated protein 1 antibody
- BPM 90 antibody
- BPM L antibody
see all
Database links
- Entrez Gene: 10048 Human
- Entrez Gene: 56705 Mouse
- Omim: 603854 Human
- SwissProt: Q96S59 Human
- SwissProt: P69566 Mouse
- Unigene: 708182 Human
- Unigene: 148781 Mouse
Anti-RanBP9 antibody images:
Western blot - RanBP9 antibody (ab64275)
All lanes : Anti-RanBP9 antibody (ab64275) at 1/2000 dilution
Lane 1 : Crude extracts of HeLa cells
Lane 2 : Crude extracts of U2OS cells.
Predicted band size : 78 kDa
Observed band size : 95 kDa (why is the actual band size different from the predicted?)
Additional bands at : 35 kDa. We are unsure as to the identity of these extra bands.
Immunocytochemistry/ Immunofluorescence - RanBP9 antibody (ab64275)
ICC/IF image of ab64275 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab64275, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-RanBP9 antibody(ab64275)
IHC image of ab64275 staining in human cervical carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab64275, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
References for Anti-RanBP9 antibody (ab64275)
ab64275 has not yet been referenced specifically in any publications.
Publishing research using ab64275? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"