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All lanes : Anti-RanGAP1 antibody - Aminoterminal end (ab59565) at 1/4000 dilution
Lane 1 : HEK-293T cells transfected with human RanGAP1
Lane 2 : Untransfected HEK-293 cells
Lane 3 : HEK-293T cells transfected with human RanGAP1 + Inhibition with RanGAP1 immunizing peptide (human 1-20) (20µg/mL)
Secondary
Goat Anti-Rabbit IgG, Peroxidase conjugate
developed using the ECL technique
Predicted band size : 64 kDa
Observed band size : 65 kDa (why is the actual band size different from the predicted?)
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