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Products:Cell Biology >> Cell Cycle >> Cell Division >> Centromere
Overview
Product name
Anti-RanGAP1 antibody - Aminoterminal end
See all RanGAP1 products (10) ...
Description
Rabbit polyclonal to RanGAP1 - Aminoterminal end
Specificity
This antibody recognizes RanGAP1.
Tested applications
ICC/IF, WBmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Chicken, Cynomolgus Monkey, Orangutan
Immunogen
Synthetic peptide: MASEDIAKLAETLAKTQVAG conjugated to KLH, corresponding to N terminal amino acids 1-20 of Human RanGAP1
MASEDIAKLA ETLAKTQVAG
Positive control
Hela cells and HEK293 T cells.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 15mM Sodium Azide
Constituents: 0.01M PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Nuclear Pore Complex
Cell Biology >> Cell Cycle >> Cell Division >> Centromere
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Western blot - RanGAP1 antibody - Aminoterminal end (ab59565)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab59565 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF
- 1 Image
WB
Application notes
ICC/IF: Use at a concentration of 10 - 20 µg/ml.
WB: Use at a concentration of 1 - 2 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 64 kDa).
Note: Staining of the RanGAP1 band in immunoblotting is specifically inhibited by the immunizing peptide.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
GTPase activator for the nuclear Ras-related regulatory protein Ran, converting it to the putatively inactive GDP-bound state.
Tissue specificity
Highly expressed in brain, thymus and testis.
Sequence similarities
Belongs to the RNA1 family.
Contains 6 LRR (leucine-rich) repeats.
Post-translational
modifications
Phosphorylated occurs before nuclear envelope breakdown and continues throughout mitosis. Phosphorylated by the M-phase kinase cyclin B/Cdk1, in vitro. Differential timimg of dephosphorylation occurs during phases of mitosis. The phosphorylated form remains associated with RANBP2/NUP358 and the SUMO E2-conjugating enzyme, UBC9, on nuclear pore complex (NPC) diassembly and during mitosis.
Sumoylated by SUMO1. Sumoylation is necessary for targeting to the nuclear envelope (NE), and for association with mitotic spindles and kinetochores during mitosis. Also required for interaction with RANBP2 and is mediated by UBC9.
Cellular localization
Cytoplasm. Nucleus membrane. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > spindle pole. Cytoplasmic during interphase. Targeted to the nuclear rim after sumoylation. During mitosis, associates with mitotic spindles. Association with kinetochores appears soon after nuclear envelope breakdown and persists until late anaphase. Mitotic location also requires sumoylation.
Target information above from: UniProt accessionP46060
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Fug 1 antibody
- Fug1 antibody
- KIAA1835 antibody
see all
Database links
- Entrez Gene: 5905 Human
- Entrez Gene: 19387 Mouse
- Entrez Gene: 362965 Rat
- Omim: 602362 Human
- SwissProt: P46060 Human
- SwissProt: P46061 Mouse
- SwissProt: Q66H32 Rat
- Unigene: 183800 Human
see all
Anti-RanGAP1 antibody - Aminoterminal end images:
Western blot - RanGAP1 antibody - Aminoterminal end (ab59565)
All lanes : Anti-RanGAP1 antibody - Aminoterminal end (ab59565) at 1/4000 dilution
Lane 1 : HEK-293T cells transfected with human RanGAP1
Lane 2 : Untransfected HEK-293 cells
Lane 3 : HEK-293T cells transfected with human RanGAP1 + Inhibition with RanGAP1 immunizing peptide (human 1-20) (20µg/mL)
Secondary
Goat Anti-Rabbit IgG, Peroxidase conjugate
developed using the ECL technique
Predicted band size : 64 kDa
Observed band size : 65 kDa (why is the actual band size different from the predicted?)
References for Anti-RanGAP1 antibody - Aminoterminal end (ab59565)
ab59565 has not yet been referenced specifically in any publications.
Publishing research using ab59565? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"