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Products:Cell Biology >> Cell Cycle >> Cell Division >> Centromere
Overview
Product name
Anti-RanGAP1 antibody
See all RanGAP1 products (10) ...
Description
Goat polyclonal to RanGAP1
Specificity
There is a hypothetical protein called "similar to RANGAP1" (XP_139737.2) that is virtually identical.
Tested applications
WB, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthetic peptide: ASEDIAKLAETLAK, corresponding to N terminal amino acids 2-15 of Human RanGAP1.
ASEDIAKLAE TLAK-C
Positive control
3T3 lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris-saline. pH 7.3
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Primary antibody notes
Ran is a small signaling GTPase that is involved in nucleocytoplasmic transport. Two additional functions of animal Ran in the formation of spindle asters and the reassembly of the nuclear envelope in mitotic cells have been recently reported. In contrast to Ras or Rho, Ran is not associated with membranes. Instead, the spatial sequestering of its accessory proteins, the Ran GTPase-activating protein RanGAP 1 and the nucleotide exchange factor RCC1, appears to define the local concentration of RanGTP vs. RanGDP involved in signaling. Mammalian RanGAP 1 is bound to the nuclear pore by a mechanism involving the attachment of small ubiquitin-related modifier protein (SUMO) to its C terminus and the subsequent binding of the SUMOylated domain to the nucleoporin Nup358.
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Nuclear Pore Complex
Cell Biology >> Cell Cycle >> Cell Division >> Centromere
Applications
Show applications key
Our Abpromise guarantee covers the use of ab2081 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
ICC/IF: 1/500.
WB: Use at a concentration of 0.1 - 0.5 µg/ml. Detects a band of approximately 70 kDa in human brain lysates and 3T3 lysates (predicted MW of 68kDa according to NP_002874).
Peptide ELISA: antibody detection limit dilution >1/32000.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
GTPase activator for the nuclear Ras-related regulatory protein Ran, converting it to the putatively inactive GDP-bound state.
Tissue specificity
Highly expressed in brain, thymus and testis.
Sequence similarities
Belongs to the RNA1 family.
Contains 6 LRR (leucine-rich) repeats.
Post-translational
modifications
Phosphorylated occurs before nuclear envelope breakdown and continues throughout mitosis. Phosphorylated by the M-phase kinase cyclin B/Cdk1, in vitro. Differential timimg of dephosphorylation occurs during phases of mitosis. The phosphorylated form remains associated with RANBP2/NUP358 and the SUMO E2-conjugating enzyme, UBC9, on nuclear pore complex (NPC) diassembly and during mitosis.
Sumoylated by SUMO1. Sumoylation is necessary for targeting to the nuclear envelope (NE), and for association with mitotic spindles and kinetochores during mitosis. Also required for interaction with RANBP2 and is mediated by UBC9.
Cellular localization
Cytoplasm. Nucleus membrane. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > spindle pole. Cytoplasmic during interphase. Targeted to the nuclear rim after sumoylation. During mitosis, associates with mitotic spindles. Association with kinetochores appears soon after nuclear envelope breakdown and persists until late anaphase. Mitotic location also requires sumoylation.
Target information above from: UniProt accessionP46060
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Fug 1 antibody
- Fug-1 antibody
- Fug1 antibody
see all
Database links
- Entrez Gene: 5905 Human
- Entrez Gene: 19387 Mouse
- Omim: 602362 Human
- SwissProt: P46060 Human
- SwissProt: P46061 Mouse
- Unigene: 183800 Human
- Unigene: 270975 Mouse
- Unigene: 475271 Mouse
Anti-RanGAP1 antibody images:
Western blot - RanGAP1 antibody (ab2081)
ab2081 at a concentration of 0.2µg/ml staining approximately 70kda RanGAP1 in 3T3 whole cell lysate (RIPA buffer, 35µg total protein per lane) by Western blot (ECL). Primary incubation = 1 hour.
Immunocytochemistry/ Immunofluorescence - RanGAP1 antibody (ab2081)
ab2081 (1/500) detecting RanGap1 in mitotic and interphase Hela cells (green). Dapi was used as counterstain in order to highlight the nucleus (blue) and cells were fixed both in Methanol (-20 °C/ 6 mins, followed by 3 washes in PBS) or Paraformaldehyde (3,7% PFA /10 min at room temperature, 0,1% Triton/PBS 5 min, glycine 5 min, followed by 3 washes in PBS); This antibody also gave good results in NIH-3T3 cells under both the above mentioned fixation procedures (data not shown).
Image courtesy of Marilena Ciciarello and Patrizia Lavia, University 'La Sapienza' CNR, Italy
References for Anti-RanGAP1 antibody (ab2081)
ab2081 has not yet been referenced specifically in any publications.
Publishing research using ab2081? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"