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Western blot - RanGAP1 antibody (ab4784)
ab4784 at a 1/500 dilution staining approximately 90kDa RanGAP 1 in Xenopus egg extract (lane X, 50
NB: RanGAP 1 in the above blot is modified by the addition of the SUMO-modifier peptide, so runs at approximately 90 kDa. The smaller band visible in the Xenopus lane at approximately 60 kDa is likely to be a small amount of non-SUMOylated protein.
Immunocytochemistry/ Immunofluorescence - RanGAP1 antibody (ab4784)
ab4784 (1/50) staining RanGap1 in mouse NIH-3T3 cells (green). Cells were fixed in methanol (-20oC/ 6 mins, followed by 3 washes in PBS) and DAPI was used as counterstain in order to highlight the nucleus (blue);
Image courtesy of Patrizia Lavia, University 'La Sapienza' CNR, Italy
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