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Products:Cell Biology >> Cell Cycle >> Cell Division >> Centromere
Overview
Product name
Anti-RanGAP1 antibody
See all RanGAP1 products (10) ...
Description
Rabbit polyclonal to RanGAP1
Tested applications
ICC/IF, WBmore details
Cross reactivity
Reacts with
Mouse, Human, Xenopus laevis
Immunogen
Synthetic peptide mixture: CHWSDMFTGRLRPEI (amino acids 82-96) and CPSPEKLVRMGPRRSA (amino acids 434-448) from Xenopus laevis
CHWSDMFTGR LRPEI and CPSPEKLVRM GPRRSA
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: PBS, Whole Serum
Purity
Whole antiserum
Primary antibody notes
Ran is a small signaling GTPase that is involved in nucleocytoplasmic transport. Two additional functions of animal Ran in the formation of spindle asters and the reassembly of the nuclear envelope in mitotic cells have been recently reported. In contrast to Ras or Rho, Ran is not associated with membranes. Instead, the spatial sequestering of its accessory proteins, the Ran GTPase-activating protein RanGAP 1 and the nucleotide exchange factor RCC1, appears to define the local concentration of RanGTP vs. RanGDP involved in signaling. Mammalian RanGAP 1 is bound to the nuclear pore by a mechanism involving the attachment of small ubiquitin-related modifier protein (SUMO) to its C terminus and the subsequent binding of the SUMOylated domain to the nucleoporin Nup358.
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Nuclear Pore Complex
Cell Biology >> Cell Cycle >> Cell Division >> Centromere
Applications
Show applications key
Our Abpromise guarantee covers the use of ab4784 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: 1/50
WB: 1/500Detects a band of approximately 60 kDa.
Target
Function
GTPase activator for the nuclear Ras-related regulatory protein Ran, converting it to the putatively inactive GDP-bound state.
Tissue specificity
Highly expressed in brain, thymus and testis.
Sequence similarities
Belongs to the RNA1 family.
Contains 6 LRR (leucine-rich) repeats.
Post-translational
modifications
Phosphorylated occurs before nuclear envelope breakdown and continues throughout mitosis. Phosphorylated by the M-phase kinase cyclin B/Cdk1, in vitro. Differential timimg of dephosphorylation occurs during phases of mitosis. The phosphorylated form remains associated with RANBP2/NUP358 and the SUMO E2-conjugating enzyme, UBC9, on nuclear pore complex (NPC) diassembly and during mitosis.
Sumoylated by SUMO1. Sumoylation is necessary for targeting to the nuclear envelope (NE), and for association with mitotic spindles and kinetochores during mitosis. Also required for interaction with RANBP2 and is mediated by UBC9.
Cellular localization
Cytoplasm. Nucleus membrane. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > spindle pole. Cytoplasmic during interphase. Targeted to the nuclear rim after sumoylation. During mitosis, associates with mitotic spindles. Association with kinetochores appears soon after nuclear envelope breakdown and persists until late anaphase. Mitotic location also requires sumoylation.
Target information above from: UniProt accessionP46060
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Fug 1 antibody
- Fug-1 antibody
- Fug1 antibody
see all
Database links
- Entrez Gene: 5905 Human
- Entrez Gene: 19387 Mouse
- Omim: 602362 Human
- SwissProt: P46060 Human
- SwissProt: P46061 Mouse
- SwissProt: O13066 Xenopus laevis
- Unigene: 183800 Human
- Unigene: 270975 Mouse
see all
Anti-RanGAP1 antibody images:
Western blot - RanGAP1 antibody (ab4784)
ab4784 at a 1/500 dilution staining approximately 90kDa RanGAP 1 in Xenopus egg extract (lane X, 50
NB: RanGAP 1 in the above blot is modified by the addition of the SUMO-modifier peptide, so runs at approximately 90 kDa. The smaller band visible in the Xenopus lane at approximately 60 kDa is likely to be a small amount of non-SUMOylated protein.
Immunocytochemistry/ Immunofluorescence - RanGAP1 antibody (ab4784)
ab4784 (1/50) staining RanGap1 in mouse NIH-3T3 cells (green). Cells were fixed in methanol (-20oC/ 6 mins, followed by 3 washes in PBS) and DAPI was used as counterstain in order to highlight the nucleus (blue);
Image courtesy of Patrizia Lavia, University 'La Sapienza' CNR, Italy
References for Anti-RanGAP1 antibody (ab4784)
This product has been referenced in:
- Xia Fet al. Tumor cell dependence on Ran-GTP-directed mitosis. Cancer Res 68:1826-33 (2008). WB; Human.Read more (PubMed: 18339863) »
- Shitashige Met al. Regulation of Wnt signaling by the nuclear pore complex. Gastroenterology 134:1961-71, 1971.e1-4 (2008).Read more (PubMed: 18439914) »
See all 3 publications for this product
Publishing research using ab4784? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"