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Western blot - RanGAP1 antibody (ab4784)

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Immunocytochemistry/ Immunofluorescence - RanGAP1 antibody (ab4784)

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Product Name
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RanGAP1 antibody
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See all RanGAP1 antibodies (7)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to RanGAP1
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Immunogen
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Synthetic peptide mixture: CHWSDMFTGRLRPEI (amino acids 82-96) and CPSPEKLVRMGPRRSA (amino acids 434-448) from Xenopus laevis BLAST 'CHWSDMFTGR LRPEI and CPSPEKLVRM ' with or 
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Reacts with
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Hu, Ms, Xl
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Tested applications
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ICC/IF, WB
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Abreviews
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Application notes
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Recommended dilutions ICC/IF: 1/50. WB: 1/500. Detects a band of approximately 60 kDa.
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Cytoplasmic
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Research areas
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Chromatin and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Nuclear Pore Complex Cell Biology >> Cell Cycle >> Cell Division >> Centromere
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Relevance
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RanGAP1 is a GTPase activator for the nuclear Ras-related regulatory protein Ran, converting it to the putatively inactive GDP-bound state.
Ran is a small signaling GTPase that is involved in nucleocytoplasmic transport. Two additional functions of animal Ran in the formation of spindle asters and the reassembly of the nuclear envelope in mitotic cells have been recently reported. In contrast to Ras or Rho, Ran is not associated with membranes. Instead, the spatial sequestering of its accessory proteins, the Ran GTPase-activating protein RanGAP 1 and the nucleotide exchange factor RCC1, appears to define the local concentration of RanGTP vs. RanGDP involved in signaling. Mammalian RanGAP 1 is bound to the nuclear pore by a mechanism involving the attachment of small ubiquitin-related modifier protein (SUMO) to its C terminus and the subsequent binding of the SUMOylated domain to the nucleoporin Nup358.
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Database links
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The links below go to external sites and will open in a new browser window
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Whole antiserum
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Storage buffer
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Preservative: 0.1% Sodium Azide Constituents: PBS, Whole Serum Material safety datasheet (MSDS) for this product: Sodium Azide MSDS
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Form
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Liquid
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Concentration
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Concentration not determined - why is this?
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this RanGAP1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for RanGAP1 antibody images, references, products related to ab4784 and other tools.
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RanGAP1 antibody images:
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Western blot - RanGAP1 antibody (ab4784)
ab4784 at a 1/500 dilution staining approximately 90kDa RanGAP 1 in Xenopus egg extract (lane X, 50µg per lane) and HeLa total cell extract (lane Hu, 50µg per lane) by western blot (ECL, 1 minute exposure). NB: RanGAP 1 in the above blot is modified by the addition of the SUMO-modifier peptide, so runs at approximately 90 kDa. The smaller band visible in the Xenopus lane at approximately 60 kDa is likely to be a small amount of non-SUMOylated protein.
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Immunocytochemistry/ Immunofluorescence - RanGAP1 antibody (ab4784)
ab4784 (1/50) staining RanGap1 in mouse NIH-3T3 cells (green). Cells were fixed in methanol (-20oC/ 6 mins, followed by 3 washes in PBS) and DAPI was used as counterstain in order to highlight the nucleus (blue); Image courtesy of Patrizia Lavia, University 'La Sapienza' CNR, Italy
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References for RanGAP1 antibody (ab4784)
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This product has been used in: (two most recent references)
Xia F et al. Tumor cell dependence on Ran-GTP-directed mitosis. Cancer Res 68:1826-33 (2008). WB; Human. PubMed: 18339863
Shitashige M et al. Regulation of Wnt signaling by the nuclear pore complex. Gastroenterology 134:1961-71, 1971.e1-4 (2008). PubMed: 18439914
See all 3 publication references for this product.
If you publish research using ab4784 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to RanGAP1
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RanGAP1 antibody - more information
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Customer reviews (feedback) regarding RanGAP1 antibody |
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RanGAP1 antibody - related products:
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RanGAP1 antibody - other tools:
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Contact Abcam with a Technical Enquiry about RanGAP1 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"