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Products:Cell Biology >> Cell Cycle >> Cell Division >> Centromere
Overview
Product name
Anti-RanGAP1 antibody
See all RanGAP1 products (10) ...
Description
Mouse polyclonal to RanGAP1
Tested applications
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse
Immunogen
Recombinant fragment: RGQGEKSATP SRKILDPNTG EPAPVLSSPP PADVSTFLAF PSPEKLLRLG PKSSVLIAQQ TDTSDPEKVV SAFLKVSSVF KDEATVRMAV QDAVDALMQK , corresponding to amino acids 401-500 of Human RanGAP1
RGQGEKSATP SRKILDPNTG EPAPVLSSPP PADVSTFLAF PSPEKLLRLG PKSSVLIAQQ TDTSDPEKVV SAFLKVSSVF KDEATVRMAV QDAVDALMQK
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 50% Glycerol, Whole serum
Purity
Whole antiserum
Primary antibody notes
This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Nuclear Pore Complex
Cell Biology >> Cell Cycle >> Cell Division >> Centromere
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Western blot - RanGAP1 antibody (ab52669)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab52669 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
WB: 1/1000. Predicted molecular weight: 64 kDa. This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
GTPase activator for the nuclear Ras-related regulatory protein Ran, converting it to the putatively inactive GDP-bound state.
Tissue specificity
Highly expressed in brain, thymus and testis.
Sequence similarities
Belongs to the RNA1 family.
Contains 6 LRR (leucine-rich) repeats.
Post-translational
modifications
Phosphorylated occurs before nuclear envelope breakdown and continues throughout mitosis. Phosphorylated by the M-phase kinase cyclin B/Cdk1, in vitro. Differential timimg of dephosphorylation occurs during phases of mitosis. The phosphorylated form remains associated with RANBP2/NUP358 and the SUMO E2-conjugating enzyme, UBC9, on nuclear pore complex (NPC) diassembly and during mitosis.
Sumoylated by SUMO1. Sumoylation is necessary for targeting to the nuclear envelope (NE), and for association with mitotic spindles and kinetochores during mitosis. Also required for interaction with RANBP2 and is mediated by UBC9.
Cellular localization
Cytoplasm. Nucleus membrane. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > spindle pole. Cytoplasmic during interphase. Targeted to the nuclear rim after sumoylation. During mitosis, associates with mitotic spindles. Association with kinetochores appears soon after nuclear envelope breakdown and persists until late anaphase. Mitotic location also requires sumoylation.
Target information above from: UniProt accessionP46060
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Fug 1 antibody
- Fug1 antibody
- KIAA1835 antibody
see all
Database links
- Entrez Gene: 5905 Human
- Entrez Gene: 19387 Mouse
- Omim: 602362 Human
- SwissProt: P46060 Human
- SwissProt: P46061 Mouse
- Unigene: 183800 Human
- Unigene: 270975 Mouse
- Unigene: 475271 Mouse
Anti-RanGAP1 antibody images:
Western blot - RanGAP1 antibody (ab52669)
All lanes : Anti-RanGAP1 antibody (ab52669) at 1/1000 dilution
Lane 1 : total protein extract from E coli with 50ng to 100 ng of a tagged fusion protein of an irrelevant antigen
Lane 2 : total protein extract from E coli with 50ng to 500ng of the tagged antigen
Secondary
Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated at 1/5000 dilution
Predicted band size : 64 kDa
Note: the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used.
References for Anti-RanGAP1 antibody (ab52669)
ab52669 has not yet been referenced specifically in any publications.
Publishing research using ab52669? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"