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Overview

  • Product nameRanGAP1 protein (Tagged-His Tag)See all RanGAP1 proteins and peptides ...
  • Protein descriptionRecombinant full length protein, corresponding to amino acids 1-357 of Human RanGAP1 with N terminal His tag. MWt 41kDa; UniProt ID = P46060
  • Expression hostE. coli

    • Properties

  • Purification notesPurity level >85%
    This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
  • FormLyophilised:Reconstitution with 98 µl aqua dest.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
  • Concentration information loading...
  • Sequence notesMASEDIAKLAETLAKTQVAGGQLSFKGKSLKLNTAEDAKD VIKEIEDFDSLEALR LEGNTVGVEAARVIAKALEKKSE LKRCHWSDMFTGRLRTEIPPALISLGEGLITA GAQLVE LDLSDNAFGPDGVQGFEALLKSSACFTLQELKLNNCGMGI GGGKILAAA LTECHRKSSAQGKPLALKVFVAGRNRLEN DGATALAEAFRVIGTLEEVHMPQNGI NHPGITALAQAF AVNPLLRVINLNDNTFTEKGAVAMAETLKTLRQVEVINFG DCL VRSKGAVAIADAIRGGLPKLKELNLSFCEIKRDAA LAVAEAMADKAELEKLDLNG NTLGEEGCEQLQEVLEGF N MAKVLASL
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab91719 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE
  • Application notesSDS-PAGE: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        Fug 1Fug1KIAA1835
        MGC20266OTTHUMP00000028918OTTHUMP00000198755OTTHUMP00000198756OTTHUMP00000198757OTTHUMP00000198758RAGP1_HUMANRan 1RAN GTPase activating protein 1Ran GTPase-activating protein 1Ran1RANGAP 1RanGAP1RANGAP1SDSegregation distorter homologSegregation distortion
      see all
  • FunctionGTPase activator for the nuclear Ras-related regulatory protein Ran, converting it to the putatively inactive GDP-bound state.
  • Tissue specificityHighly expressed in brain, thymus and testis.
  • Sequence similaritiesBelongs to the RNA1 family.
    Contains 6 LRR (leucine-rich) repeats.
  • Post-translational
    modifications    Phosphorylated occurs before nuclear envelope breakdown and continues throughout mitosis. Phosphorylated by the M-phase kinase cyclin B/Cdk1, in vitro. Differential timimg of dephosphorylation occurs during phases of mitosis. The phosphorylated form remains associated with RANBP2/NUP358 and the SUMO E2-conjugating enzyme, UBC9, on nuclear pore complex (NPC) diassembly and during mitosis.
    Sumoylated by SUMO1. Sumoylation is necessary for targeting to the nuclear envelope (NE), and for association with mitotic spindles and kinetochores during mitosis. Also required for interaction with RANBP2 and is mediated by UBC9.
  • Cellular localizationCytoplasm. Nucleus membrane. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > spindle pole. Cytoplasmic during interphase. Targeted to the nuclear rim after sumoylation. During mitosis, associates with mitotic spindles. Association with kinetochores appears soon after nuclear envelope breakdown and persists until late anaphase. Mitotic location also requires sumoylation.

    • Target information above from: UniProt accession P46060 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    RanGAP1 protein (Tagged-His Tag) images

    • SDS-PAGE - RanGAP1 protein (Tagged-His Tag) (ab91719)
      SDS-PAGE - RanGAP1 protein (Tagged-His Tag) (ab91719)
      The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images coloured control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.

    References for RanGAP1 protein (Tagged-His Tag) (ab91719)

    ab91719 has not yet been referenced specifically in any publications.

    Publishing research using ab91719 ? Please let us know so that we can cite the reference in this datasheet

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