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Product name
Raptor peptide
See all Raptor products (4) ...
Protein description
Synthetic peptide, corresponding to the N-Terminus of human Raptor.
Properties
Purification notes
ab39860 is a crude synthetic peptide, purity range from 60-70%. The sequence is proprietary.
Biological activity
This peptide is used for blocking the activity of the Anti-Raptor antibody (ab26264).
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.1% BSA, PBS, pH 7.2
Concentration
Concentration information loading...
Research areas
Epigenetics and Nuclear Signaling >> Cell cycle >> Cell Cycle Inhibitors >> Other
Signal Transduction >> Adapters >> Cytoplasmic
Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> Other
Applications
Show applications key
Our Abpromise guarantee covers the use of ab39860 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Blocking
Application notes
BL: Use at an assay dependent dilution.
This peptide can be used with studies using ab26264.
This peptide usually blocks the antibody activity completely in Western blot by incubating the peptide with an equal volume of antibody for 30 min at 37°C.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Protein info
Function
Involved in the control of the mammalian target of rapamycin complex 1 (mTORC1) activity which regulates cell growth and survival, and autophagy in response to nutrient and hormonal signals; functions as a scaffold for recruiting mTORC1 substrates. mTORC1 is activated in response to growth factors or amino-acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino-acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation.
Tissue specificity
Highly expressed in skeletal muscle, and in a lesser extent in brain, lung, small intestine, kidney and placenta.
Sequence similarities
Belongs to the WD repeat RAPTOR family.
Contains 7 WD repeats.
Cellular localization
Cytoplasm. Lysosome. Targeting to lysosomes depends on amino acid availability.
Target information above from: UniProt accessionQ8N122
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
References for Raptor peptide (ab39860)
ab39860 has not yet been referenced specifically in any publications.
Publishing research using ab39860? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"