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RabMAbs (1819-1)

Anti-Ras antibody [EP1125Y] (ab52939)

Abreviews (1)Q&A (3)Specific References (2)

Overview

  • Product nameAnti-Ras antibody [EP1125Y]See all Ras primary antibodies ...
  • Description
    Rabbit monoclonal [EP1125Y] to Ras
  • Specificityab52939 is predicted to react with H-Ras, N-Ras and K-Ras.
  • Tested applicationsWB, IP, Flow Cyt more details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues near the N-terminal region of human Ras.

  • Positive controlC6 cell lysate and permeabilized PC-12 cells.
  • General notesProduced under U.S. Patent No. 5,675,063.

Applications

Our Abpromise guarantee covers the use of ab52939 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB
IP
Flow Cyt
  • Application notesFlow Cyt: 1/100.
    IP: 1/30.
    WB: 1/500000. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa).

    Is unsuitable for ICC or IHC.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • FunctionRas proteins bind GDP/GTP and possess intrinsic GTPase activity.
    • Involvement in diseaseDefects in HRAS are the cause of faciocutaneoskeletal syndrome (FCSS) [MIM:218040]. A rare condition characterized by prenatally increased growth, postnatal growth deficiency, mental retardation, distinctive facial appearance, cardiovascular abnormalities (typically pulmonic stenosis, hypertrophic cardiomyopathy and/or atrial tachycardia), tumor predisposition, skin and musculoskeletal abnormalities.
      Defects in HRAS are the cause of congenital myopathy with excess of muscle spindles (CMEMS) [MIM:218040]. CMEMS is a variant of Costello syndrome.
      Defects in HRAS may be a cause of susceptibility to Hurthle cell thyroid carcinoma (HCTC) [MIM:607464]. Hurthle cell thyroid carcinoma accounts for approximately 3% of all thyroid cancers. Although they are classified as variants of follicular neoplasms, they are more often multifocal and somewhat more aggressive and are less likely to take up iodine than are other follicular neoplasms.
      Note=Mutations which change positions 12, 13 or 61 activate the potential of HRAS to transform cultured cells and are implicated in a variety of human tumors.
      Defects in HRAS are a cause of susceptibility to bladder cancer (BLC) [MIM:109800]. A malignancy originating in tissues of the urinary bladder. It often presents with multiple tumors appearing at different times and at different sites in the bladder. Most bladder cancers are transitional cell carcinomas. They begin in cells that normally make up the inner lining of the bladder. Other types of bladder cancer include squamous cell carcinoma (cancer that begins in thin, flat cells) and adenocarcinoma (cancer that begins in cells that make and release mucus and other fluids). Bladder cancer is a complex disorder with both genetic and environmental influences.
      Note=Defects in HRAS are the cause of oral squamous cell carcinoma (OSCC).
    • Sequence similaritiesBelongs to the small GTPase superfamily. Ras family.
    • Post-translational
      modifications      Palmitoylated by the ZDHHC9-GOLGA7 complex. A continuous cycle of de- and re-palmitoylation regulates rapid exchange between plasma membrane and Golgi.
      S-nitrosylated; critical for redox regulation. Important for stimulating guanine nucleotide exchange. No structural perturbation on nitrosylation.
    • Cellular localizationCell membrane. Golgi apparatus membrane. The active GTP-bound form is localized most strongly to membranes than the inactive GDP-bound form (By similarity). Shuttles between the plasma membrane and the Golgi apparatus.

      • Target information above from: UniProt accession P01112 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
      see all
    • Alternative names
        c bas/has antibodyc H ras antibodyc has/bas p21 protein antibody
        C K RAS antibodyc K ras2 protein antibodyc Ki ras antibodyc Kirsten ras protein antibodyc ras Ki 2 protein antibodyC-BAS/HAS antibodyc-H-ras antibodyC-HA-RAS1 antibodyCellular c Ki ras2 proto oncogene antibodyCTLO antibodyG1III6 N ras antibodyGTPase HRas antibodyGTPase KRas antibodyGTPase NRas antibodyH Ras 1 antibodyH RasIDX antibodyH-Ras-1 antibodyH-RASIDX antibodyHa Ras antibodyHa-Ras antibodyHAMSV antibodyHRAS 1 antibodyHRAS antibodyHRAS1 antibodyK Ras 2 antibodyK ras antibodyK ras p21 protein antibodyK RAS2A antibodyK RAS2B antibodyK RAS4A antibodyK RAS4B antibodyK-RAS antibodyKI RAS antibodyKirsten rat sarcoma 2 viral (v Ki ras2) oncogene homolog antibodyKRAS 1 antibodyKRAS 2 antibodyKRAS antibodyKRAS1 antibodyKRAS2 antibodyN ras antibodyN ras oncogene antibodyN-RAS antibodyN-terminally processed antibodyNeuroblastoma RAS viral (v ras) oncogene homolog antibodyNRAS 1 antibodyNRAS antibodyNRAS1 antibodyNS3 antibodyOncogene KRAS2 antibodyp21 protein antibodyp21ras antibodyPR310 c K ras oncogene antibodyRASH 1 antibodyRASH_HUMAN antibodyRASH1 antibodyRASK 2 antibodyRASK2 antibodyTransforming protein N Ras antibodyTransforming protein p21 antibodyv Ha ras Harvey rat sarcoma viral oncogene homolog antibodyv Ki ras2 Kirsten rat sarcoma 2 viral oncogene homolog antibodyv Ki ras2 Kirsten rat sarcoma viral oncogene homolog antibodyv ras neuroblastoma RAS viral oncogene homolog antibody
      see all

    Anti-Ras antibody [EP1125Y] images

    • Western blot - Ras antibody [EP1125Y] (ab52939)
      Western blot - Ras antibody [EP1125Y] (ab52939)
      Anti-Ras antibody [EP1125Y] (ab52939) at 1/500000 dilution + C6 cell lysate at 10 µg/ml

      Secondary
      goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 18 kDa
      Observed band size : 18 kDa
    • Flow Cytometry - Ras antibody [EP1125Y] (ab52939)
      Flow Cytometry - Ras antibody [EP1125Y] (ab52939)
      ab52939 at 1/100 dilution staining Ras in permeabilized PC-12 cells by flow cytometry (red). Rabbit IgG negative control (green).
    • Western blot
      Western blot
      Anti-Ras antibody [EP1125Y] (ab52939) at 1/500 dilution + Ras protein (ab56526) at 0.01 µg

      Secondary
      Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
      developed using the ECL technique

      Performed under reducing conditions.

      Exposure time : 1 minute

    References for Anti-Ras antibody [EP1125Y] (ab52939)

    This product has been referenced in:
    • Maurer T  et al. Small-molecule ligands bind to a distinct pocket in Ras and inhibit SOS-mediated nucleotide exchange activity. Proc Natl Acad Sci U S A 109:5299-304 (2012). WB ; Human . Read more (PubMed: 22431598) »
    • Johnston M  et al. HSP90 protein stabilizes unloaded argonaute complexes and microscopic P-bodies in human cells. Mol Biol Cell 21:1462-9 (2010). WB ; Human . Read more (PubMed: 20237157) »

    See all 2 Publications for this product

    Publishing research using ab52939 ? Please let us know so that we can cite the reference in this datasheet

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    Thank you for your assistance.

    Please see below details :

    Original order number #####

    1 Xvial of ab52939

    Original order number: ####
    1 X vial ab32072

    Looking forward towards your valued response...

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    Thank you for kindly confirming these details. I am sorry these antibodies have not worked for this customer.

    As requested, we are pleased to arrange the alternative free of charge replacements. I can confirm that 1 vial of ab87980 and 1 vial o...

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    Dear Technical Team,

    Hope this e-mail finds you well!

    Please refer to the below mail from one of our customer at AIIMS- New Delhi to whom we recently supplied two FOCR viz. ab52939 & ab32072. Again
    this customer is not getti...

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    Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear the customer hashad difficulty obtaining satisfactory results from the free of charge replacement vials.

    The details you have kindly provided will pr...

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    Western blot abreview for Anti-Ras antibody [EP1125Y]

     Excellent
    Abreviews
    Application Western blot
    Sample Human Cell lysate - whole cell (A549)
    Loading amount 50 µg
    Specification A549
    Gel Running Conditions Reduced Denaturing (10% SDS-PAGE)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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    Verified customer

    Submitted Sep 17 2010

    Hello again. I am interested in purchasing this product, but I do not want to do that until I understand how your results were obtained. The only flow data available to consumers is the single graph. Is there anymore information that can be shared?　I d...

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    Thank you for your enquiry. I have obtained some further information on the flow cytometry protocol used to test this antibody. 2% paraformaldehyde was used for fixation: Solutions and Reagents: 1. 1X PBS 2. Blocking buffer 0.5% BSA in 1X PBS 3....

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