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Rabbit polyclonal Secondary Antibody to Rat IgG+IgM+IgA (FITC) (ab8520)

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Overview

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C in the dark. Do not freeze.
  • Storage bufferPreservative: None
    Constituents: 50% Glycerol, 0.1M Sodium chloride, 0.01M Sodium phosphate, pH 7.5
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesSpecific antibodies were absorpted by incubation with Sepharose-bound rat immunoglobulins. Specific antibodies were eluted by acidic buffer at pH 2.5 followed by neutralisation and dialysis. After repeated binding with immobilized rat immunoglobulins a minimum of 65% protein bound.
  • Conjugation notesThe purified polyclonal was conjugated to fluorescein isothiocyanate isomer I followed by gel-filtration and ion-exchange chromatography to clear unbound conjugate.
  • Clonality Polyclonal
  • General notesF/P ratio is 3-5 mol Fluorescein per 1 mol of rabbit Ig. There is low background fluorescence of normal tissue in contrast to specific staining of sections treated with monoclonal rat anti-human keratin 18 antibodies
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab8520 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Flow Cyt Flow Cyt: 1/40.
ICC/IF ICC/IF: Use at an assay dependent concentration.

References for Rabbit polyclonal Secondary Antibody to Rat IgG+IgM+IgA (FITC) (ab8520)

ab8520 has not yet been referenced specifically in any publications.

Publishing research using ab8520 ? Please let us know so that we can cite the reference in this datasheet

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Submit an Abreview for ab8520 Submit a Question for ab8520

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Dear Dr. Campbell, I think you wrote my claim perfectly, and you understood my question fully. Thank you for your attention. To answer your question about if I used rabbit serum in my blocking step, I say, I used 2% BSA and 0.1% triton X20, I also ...

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Thank you for your enquiry. I have contacted the originator of the antibody regarding your question and it is true, this antibody to rat immunoglobulin cross-reacts to mouse IgG. I have updated our datasheet to reflect this and I can certainly issue y...

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BATCH NUMBER 108282 ORDER NUMBER -- NOT SPECIFIED -- DESCRIPTION OF THE PROBLEM Non-specific staining and low signal SAMPLE Human chorionic villi sample and hESC PRIMARY ANTIBODY [competitor]/rat SSEA-3 IgM/blocking solution/1:5...

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Thank you for your enquiry. May I also take the time to thank you for filling in your questionnaire so comprehensively. I am sorry to hear that you have been obtaining high background with this antiserum. Given that you mention in your questionnaire th...

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I would like to know if this antibody recognizes equally well rat IgG2a or IgG2b isotypes. Thanks.

Yes, the Rabbit anti-Rat IgG,M,A recognizes the rat IgG2a and IgG2b equally.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"