Goat F(ab) polyclonal Secondary Antibody to Rat IgG - H&L (HRP) (ab7177)
Overview
- Product nameGoat F(ab) polyclonal Secondary Antibody to Rat IgG - H&L (HRP)See all IgG secondary antibodies ...
- Target speciesRat
- SpecificityAssay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Goat Serum. No reaction was observed against anti-Papain or anti-Goat IgG F(c).
- Tested applicationsICC/IF, WB, ELISA, Electron Microscopy, IHC, Dot Blot more details
- Immunogen
Rat IgG whole molecule
Properties
- FormLiquid
- Storage instructionsStore at +4°C in the dark. Do not freeze.
- Storage bufferPreservative: 0.01% Gentamicin Sulfate
Constituents: 10mg/ml BSA (IgG and protease free), 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2 -
Concentration information loading... - Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation.
- Conjugation notesHorseradish Peroxidase (HRP) (Molecular Weight 40,000 daltons)
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab7177 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| ICC/IF | ICC/IF: 1/500 - 1/2500. |
| WB | WB: Use at an assay dependent dilution. |
| ELISA | ELISA: 1/10000 - 1/40000. |
| Electron Microscopy | EM: Use at an assay dependent dilution. |
| IHC | IHC: Use at an assay dependent dilution. |
| Dot Blot | Dot: Use at an assay dependent dilution. |
References for Goat F(ab) polyclonal Secondary Antibody to Rat IgG - H&L (HRP) (ab7177)
ab7177 has not yet been referenced specifically in any publications.
