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Goat anti-Rat IgG H&L (Cy5 ®) secondary antibody (ab6565)

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Overview

Product name

Goat anti-Rat IgG H&L (Cy5 ®) secondary antibody
See all IgG products (1693) ...

Description

Goat polyclonal Secondary Antibody to Rat IgG - H&L (Cy5 ®), pre-adsorbed

Target species

Rat

Tested applications

IF, ICC, Flow Cyt, ICC/IF, ELISA, IHC-P, IHC-Frmore details

Minimal
cross-reactivity


Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rabbit, Sheepmore details

Immunogen

Rat IgG, whole molecule (H&L)

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

0.01M Na phosphate, 0.25M NaCl, pH7.6, 0.05% Na azide, 0.01% Thimerisol, 15mg/ml BSA

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Purification notes

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Conjugation notes

Cy5.29 (Cyanine 5.29-OSu) (Molecular Weight 975 daltons) Absorption Wavelength: 650 nm Emission Wavelength: 667 nm Fluorochrome/Protein Ratio: 6.0 moles Cy5 per mole of Goat IgG

Clonality

Polyclonal

General notes

This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited. This material is also subject to proprietary rights of GE Healthcare Bio-Sciences Corp. and Carnegie Mellon University and made and sold under License from GE Healthcare Bio-Sciences Corp. This product is licensed for sale only for research. It is NOT licensed for any other use. There is no implied license hereunder for any commercial use. COMMERCIAL USE shall include: 1 Sale, lease, license or other transfer of the material or any material derived or produced from it. 2 Sale, lease, license or other grant of rights to use this material or any material derived or produced from it. 3 Use of this material to perform services for a fee for third parties. If you require a commercial license to use this material and do not have one, please return this material, unopened to Abcam Plc of 330 Cambridge Science Park, Cambridge, CB4 0FL, and any money paid for the material will be refunded.

Research areas

Secondary antibodies >> anti-Rat >> IgG >> Fluorophore >> Cy5
Immunology >> Immunoglobulins >> Heavy Chain >> IgG

  • Immunocytochemistry/ Immunofluorescence - Goat polyclonal Secondary Antibody to Rat IgG - H&L (Cy5 ®), pre-adsorbed (ab6565)Immunocytochemistry/ Immunofluorescence - Goat polyclonal Secondary Antibody to Rat IgG - H&L (Cy5 ®), pre-adsorbed (ab6565) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab6565 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Goat anti-Rat IgG H&L (Cy5 ®) secondary antibody images:

  Immunocytochemistry/ Immunofluorescence - Goat polyclonal Secondary Antibody to Rat IgG - H&L (Cy5 ®), pre-adsorbed (ab6565)

Immunocytochemistry/ Immunofluorescence - Goat polyclonal Secondary Antibody to Rat IgG - H&L (Cy5 ®), pre-adsorbed (ab6565)

Ab6565 was used at dilution 1/500 with the primary antibody ab6161 in ICC/IF. See the review on ab6161.

References for Goat anti-Rat IgG H&L (Cy5 ®) secondary antibody (ab6565)

This product has been referenced in:

  • Minczuk Met al. TEFM (c17orf42) is necessary for transcription of human mtDNA. Nucleic Acids Res 39:4284-4299 (2011). ICC/IF.Read more (PubMed: 21278163) »
  • Johnson LAet al. Apolipoprotein E4 Exaggerates Diabetic Dyslipidemia and Atherosclerosis in Mice Lacking the LDL Receptor. Diabetes 60:2285-94 (2011). IHC-FoFr.Read more (PubMed: 21810592) »

See all 4 publications for this product

Publishing research using ab6565? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"