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Rabbit polyclonal Secondary Antibody to Rat IgG - H&L (Biotin) (ab6733)

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Overview

Product name

Rabbit polyclonal Secondary Antibody to Rat IgG - H&L (Biotin)
See all IgG products (1691) ...

Target species

Rat

Tested applications

WB, ELISA, Immunomicroscopy, Dot Blot, ICC/IF, IHC-P, IHC-Frmore details

Immunogen

Rat IgG whole molecule

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

0.02M K Phosphate, 0.15M NaCl, 0.01% Sodium Azide, pH7.2

Concentration

Concentration information loading...

Purity

IgG fraction

Purification notes

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Conjugation notes

Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Rabbit IgG molecule

Clonality

Polyclonal

  • Immunohistochemistry (Paraffin-embedded sections) - Rat IgG antibody - H&L (ab6733)Immunohistochemistry (Paraffin-embedded sections) - Rat IgG antibody - H&L (ab6733) image (enlarge)

  • Immunohistochemistry (Frozen sections) - Rabbit polyclonal Secondary Antibody to Rat IgG - H&L (Biotin) (ab6733)Immunohistochemistry (Frozen sections) - Rabbit polyclonal Secondary Antibody to Rat IgG - H&L (Biotin) (ab6733) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab6733 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • WB

     WB: 1/2000 - 1/10000.

    WB: 1/2000 - 1/10000.

  • ELISA

     ELISA: 1/500000

    ELISA: 1/500000

  • ShowHide

    Immunomicroscopy

     IM: Use at an assay depe...Read more →

    IM: Use at an assay dependent concentration.

  • ShowHide

    Dot Blot

     Dot: Use at an assay dep...Read more →

    Dot: Use at an assay dependent concentration.

  • ICC/IF

     ICC/IF: 1/1000 - 1/5000.

    ICC/IF: 1/1000 - 1/5000.

  • ShowHide1 Image

    IHC-P

     IHC-P: Use at an assay d...Read more →

    IHC-P: Use at an assay dependent concentration.

  • ShowHide1 Image

    IHC-Fr

     IHC-Fr: Use at an assay ...Read more →

    IHC-Fr: Use at an assay dependent concentration.

Rabbit polyclonal Secondary Antibody to Rat IgG - H&L (Biotin) images:

  Immunohistochemistry (Paraffin-embedded sections) - Rat IgG antibody - H&L (ab6733)

Immunohistochemistry (Paraffin-embedded sections) - Rat IgG antibody - H&L (ab6733)

Ab6640 (Rat monoclonal [CI:A3-1] to F4/80) at a 1/8000 dilution staining mouse spleen tissue by IHC-P. Ab6733 was used as the secondary antibody at a dilution of 1/400.

This image is courtesy of an Abreview submitted by Dr Jiqiang Zhang

See Abreview

  Immunohistochemistry (Frozen sections) - Rabbit polyclonal Secondary Antibody to Rat IgG - H&L (Biotin) (ab6733)

Immunohistochemistry (Frozen sections) - Rabbit polyclonal Secondary Antibody to Rat IgG - H&L (Biotin) (ab6733)

Ab6733 was used at dilution 1/100 with the primary antibody ab25375 in IHC-Fr. See the review on ab25375.

References for Rabbit polyclonal Secondary Antibody to Rat IgG - H&L (Biotin) (ab6733)

This product has been referenced in:

  • Lee HJet al. Oral administration of penta-O-galloyl-{beta}-D-glucose suppresses triple-negative breast cancer xenograft growth and metastasis in strong association with JAK1-STAT3 inhibition. Carcinogenesis 32:804-11 (2011). IHC.Read more (PubMed: 21289371) »
  • Latimer CSet al. Reversal of glial and neurovascular markers of unhealthy brain aging by exercise in middle-aged female mice. PLoS One 6:e26812 (2011). IHC-Fr.Read more (PubMed: 22046366) »

See all 5 publications for this product

Publishing research using ab6733? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"