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Products:Immunology >> Immunoglobulins >> Heavy Chain >> IgG
Overview
Product name
Rabbit polyclonal Secondary Antibody to Rat IgG - H&L (Biotin)
See all IgG products (1691) ...
Target species
Rat
Tested applications
WB, ELISA, Immunomicroscopy, Dot Blot, ICC/IF, IHC-P, IHC-Frmore details
Immunogen
Rat IgG whole molecule
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
0.02M K Phosphate, 0.15M NaCl, 0.01% Sodium Azide, pH7.2
Concentration
Concentration information loading...
Purity
IgG fraction
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Conjugation notes
Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Rabbit IgG molecule
Clonality
Polyclonal
Research areas
Secondary antibodies >> anti-Rat >> IgG >> Biotin
Immunology >> Immunoglobulins >> Heavy Chain >> IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab6733 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
WB: 1/2000 - 1/10000.WB: 1/2000 - 1/10000.
ELISA
ELISA: 1/500000ELISA: 1/500000
IM: Use at an assay dependent concentration.
Dot: Use at an assay dependent concentration.
ICC/IF
ICC/IF: 1/1000 - 1/5000.ICC/IF: 1/1000 - 1/5000.
IHC-P: Use at an assay dependent concentration.
IHC-Fr: Use at an assay dependent concentration.
Rabbit polyclonal Secondary Antibody to Rat IgG - H&L (Biotin) images:
Immunohistochemistry (Paraffin-embedded sections) - Rat IgG antibody - H&L (ab6733)
Ab6640 (Rat monoclonal [CI:A3-1] to F4/80) at a 1/8000 dilution staining mouse spleen tissue by IHC-P. Ab6733 was used as the secondary antibody at a dilution of 1/400.
This image is courtesy of an Abreview submitted by Dr Jiqiang Zhang
Immunohistochemistry (Frozen sections) - Rabbit polyclonal Secondary Antibody to Rat IgG - H&L (Biotin) (ab6733)
Ab6733 was used at dilution 1/100 with the primary antibody ab25375 in IHC-Fr. See the review on ab25375.
References for Rabbit polyclonal Secondary Antibody to Rat IgG - H&L (Biotin) (ab6733)
This product has been referenced in:
- Lee HJet al. Oral administration of penta-O-galloyl-{beta}-D-glucose suppresses triple-negative breast cancer xenograft growth and metastasis in strong association with JAK1-STAT3 inhibition. Carcinogenesis 32:804-11 (2011). IHC.Read more (PubMed: 21289371) »
- Latimer CSet al. Reversal of glial and neurovascular markers of unhealthy brain aging by exercise in middle-aged female mice. PLoS One 6:e26812 (2011). IHC-Fr.Read more (PubMed: 22046366) »
See all 5 publications for this product
Publishing research using ab6733? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"