|
|
|
|
Immunohistochemistry (Paraffin-embedded sections) - Rat IgG antibody - H&L (ab6733)
(enlarge)
|
|
|
|
|
Product Name
|
Rat IgG secondary antibody - H&L
|
|
Product type
|
Secondary antibodies
|
|
Description
|
Rabbit polyclonal to Rat IgG - H&L (Biotin)
|
|
Immunogen
|
Rat IgG whole molecule
|
|
Tested applications
(see key)
|
Dot, ELISA, IHC-Fr, IHC-P, IM, WB
|
|
Application notes
(see key)
|
Recommended dilutions
immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency
Please refer to the relevant conjugation for dilutions specific for this product:
Conjugate |
ELISA |
Blotting |
Flow Cytometry |
IHC |
Fluorescein |
nd |
nd |
50 - 200 |
200 - 1,000 |
Rhodamine, Texas RedTM |
nd |
nd |
50 - 200 |
200 - 1,000 |
R-Phycoerythrin |
nd |
nd |
100 - 200 |
nd |
Cyanines |
nd |
nd |
400 |
100 - 1,000 |
Biotin |
5,000 - 20,000 |
3,000 - 10,000 |
nd |
100 - 1,000 |
Peroxidase |
4,000 - 10,000 |
2,000 - 5,000 |
nd |
200 - 2,000 |
Alkaline Phosphatase |
1,000 - 2,000 |
500 - 1,000 |
nd |
100 - 1,000 |
|
|
|
Cellular localization
|
Secreted
|
|
Research areas
|
Secondary antibodies >> anti-Rat >> IgG >> Biotin
Immunology >> Immunoglobulins >> Heavy Chain >> IgG
|
|
Relevance
|
Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/ml in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.
|
|
|
|
|
Raised in
|
Rabbit
|
|
Clonality
|
Polyclonal
|
|
Purity
|
IgG fraction
|
|
Conjugation notes
|
Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC)
Biotin/Protein Ratio: 10-20 BAC molecules per Rabbit IgG molecule
|
|
Storage buffer
|
0.02M K Phosphate, 0.15M NaCl, 0.01% Sodium Azide, pH7.2 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
|
|
Purification notes
|
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
|
|
Form
|
Liquid
|
|
Conjugation
|
Biotin
|
|
Concentration
|
2.000 mg/ml
|
|
Storage instructions
|
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Rat IgG secondary antibody - H&L is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
| |
Rat IgG secondary antibody - H&L images:
|
 Immunohistochemistry (Paraffin-embedded sections) - Rat IgG antibody - H&L (ab6733)
Ab6640 (Rat monoclonal [CI:A3-1] to F4/80) at a 1/8000 dilution staining mouse spleen tissue by IHC-P. Ab6733 was used as the secondary antibody at a dilution of 1/400. This image is courtesy of an Abreview submitted by Dr Jiqiang Zhang
See Abreview
|
| |
References for Rat IgG secondary antibody - H&L (ab6733)
|
|
This product has been used in: (two most recent references)
Modica S et al. The intestinal nuclear receptor signature with epithelial localization patterns and expression modulation in tumors. Gastroenterology 138:636-48, 648.e1-12 (2010). IHC-P; Human. PubMed: 19818784
Mecha M et al. Expression of TGF-betas in the embryonic nervous system: analysis of interbalance between isoforms. Dev Dyn 237:1709-17 (2008). PubMed: 18498095
If you publish research using ab6733 please let us know so that we can cite the reference on this datasheet.
|
| |
|
Search PubMed (MEDLINE) for references to IgG
|
| |
Rat IgG secondary antibody - H&L - more information
|
Customer reviews (feedback) regarding Rat IgG secondary antibody - H&L |
Customer FAQs regarding Rat IgG secondary antibody - H&L |
Protocols for Rat IgG secondary antibody - H&L |
Price and availability of products related to Rat IgG secondary antibody - H&L |
| |
Rat IgG secondary antibody - H&L - other tools:
|
Contact Abcam with a Technical Enquiry about Rat IgG secondary antibody - H&L |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
