Rat IgG secondary antibody - H&L (ab6734)

Product Name 

Rat IgG secondary antibody - H&L

Product type 

Secondary antibodies

Description 

Rabbit polyclonal to Rat IgG - H&L (HRP)

Immunogen 

Rat IgG whole molecule

Tested applications 
(see key)

Dot, ELISA, IHC-Fr, IHC-P, IM, WB

Application notes 
(see key)

Recommended dilutions
WB: Use at an assay dependent concentration (from PubMed:16242076).
suitable for immunoblotting (dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency

Please refer to the relevant conjugation for dilutions specific for this product:

Cellular localization 

Secreted

Research areas 

Secondary antibodies >> anti-Rat >> IgG >> Enzyme >> HRP
Immunology >> Immunoglobulins >> Heavy Chain >> IgG

Relevance 

Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/ml in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 10¹¹ variants.

Raised in 

Rabbit

Clonality 

Polyclonal

Purity 

IgG fraction

Conjugation notes 

Horseradish Peroxidase (HRP)

Storage buffer 

Preservative: 0.01% Gentamicin Sulfate
Constituents: 10mg/ml BSA, 0.15M Sodium chloride, 0.02M Potassium phosphate, pH 7.2

Purification notes 

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Form 

Liquid

Conjugation 

Horse Radish Peroxidase

Concentration 

2.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

References for Rat IgG secondary antibody - H&L (ab6734)

  This product has been used in: (two most recent references)

Cutress ML et al. Structural basis for the nuclear import of the human androgen receptor. J Cell Sci 121:957-68 (2008). WB. PubMed: 18319300

Lim JC et al. Activation of beta-catenin signalling by GSK-3 inhibition increases p-glycoprotein expression in brain endothelial cells. J Neurochem 106:1855-65 (2008). WB. PubMed: 18624906

See all 4 publication references for this product.


If you publish research using ab6734 please let us know so that we can cite the reference on this datasheet.

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