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Sheep polyclonal Secondary Antibody to Rat IgG - H&L (Biotin) (ab6851)

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Overview

  • Product nameSheep polyclonal Secondary Antibody to Rat IgG - H&L (Biotin)See all IgG secondary antibodies ...
  • Target speciesRat
  • Tested applicationsWB, ELISA, Immunomicroscopy, Dot Blot, ICC/IF, IHC-P, IHC-Fr more details
  • Immunogen

    Rat IgG whole molecule

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer0.01M Na phosphate, 0.25M NaCl, pH7.6, 0.05% Na azide, 0.01% Thimerisol, 15mg/ml BSA
  • Concentration information loading...
  • PurityIgG fraction
  • Purification notesBiotinamidocaproate N-Hydroxysuccinimide Ester (BAC). Biotin/Protein Ratio: 10-20 BAC molecules per Rabbit IgG molecule.
  • Conjugation notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities
  • Clonality Polyclonal
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab6851 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB
ELISA
Immunomicroscopy
Dot Blot
ICC/IF
IHC-P
IHC-Fr
  • Application notesimmunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency

    • Sheep polyclonal Secondary Antibody to Rat IgG - H&L (Biotin) images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Sheep polyclonal Secondary Antibody to Rat IgG - H&L (Biotin) (ab6851)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Sheep polyclonal Secondary Antibody to Rat IgG - H&L (Biotin) (ab6851)
      Ab6851 was used at dilution 1/100 with the primary antibody ab56299 in IHC-P. See the review on ab56299.

    References for Sheep polyclonal Secondary Antibody to Rat IgG - H&L (Biotin) (ab6851)

    ab6851 has not yet been referenced specifically in any publications.

    Publishing research using ab6851 ? Please let us know so that we can cite the reference in this datasheet

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"