Rat IgG secondary antibody - H&L (ab7010)

Product Name 

Rat IgG secondary antibody - H&L

Product type 

Secondary antibodies

Description 

Goat polyclonal to Rat IgG - H&L (PE)

Immunogen 

Full length native Rat IgG (purified).

Species Reactivity 

Minimum reactivity to Bovine, Horse, Human, Mouse, Rabbit and Sheep Serum Proteins.

Tested applications 
(see key)

Flow Cyt, IHC-Fr, IHC-P, IM

Application notes 
(see key)

Recommended dilutions
Flow Cyt: Use 1 x 10⁶ cells in flow cytometry is approximately 1.0 µg of antibody conjugate.
IHC-P: Use at an assay dependant concentration.
IHC-Fr: Use at an assay dependant concentration.
IM: Use at an assay dependant concentration.

As a general guideline, dilutions of 1:50 to 1:200 should be suitable for most applications.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Please refer to the relevant conjugation for dilutions specific for this product:

Cellular localization 

Secreted

Research areas 

Secondary antibodies >> anti-Rat >> IgG >> Fluorophore >> Phycoerythrin
Immunology >> Immunoglobulins >> Heavy Chain >> IgG

Relevance 

Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/ml in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 10¹¹ variants.

Can be used in immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

Raised in 

Goat

Clonality 

Polyclonal

Purity 

Immunogen affinity purified

Conjugation notes 

R-Phycoerythrin (R-PE) Molecular Weight 240,000 daltons)
Absorption Wavelength: 488 nm
Emission Wavelength: 575 nm
OD_566nm/OD_280nm: 2.8

Storage buffer 

Preservative: 0.01% Sodium Azide
Constituents: 10mg/ml BSA, 0.125M Sodium Borate, 0.075M Sodium Chloride, 0.005M EDTA. pH 8

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Purification notes 

Prepared from monospecific antiserum by immunoaffinity chromatography using rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation

Form 

Liquid

Conjugation 

Phycoerythrin

Concentration 

0.500 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Notes 

This antibody is a F(ab')₂ fragment.

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