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Products:Immunology >> Immunoglobulins >> Heavy Chain >> IgG
Overview
Product name
Goat anti-Rat IgG H&L (PE) secondary antibody
See all IgG products (1691) ...
Description
Goat F(ab')2 polyclonal Secondary Antibody to Rat IgG - H&L (PE), pre-adsorbed
Target species
Rat
Tested applications
ICC/IF, IHC-P, IHC-Fr, Immunomicroscopy, Flow Cytmore details
Minimal
cross-reactivity
Cow, Horse, Human, Mouse, Rabbit, Sheepmore details
Immunogen
Full length native Rat IgG (purified).
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.01% Sodium Azide
Constituents: 10mg/ml BSA, 0.125M Sodium Borate, 0.075M Sodium Chloride, 0.005M EDTA. pH 8
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Prepared from monospecific antiserum by immunoaffinity chromatography using rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation
Conjugation notes
R-Phycoerythrin (R-PE) Molecular Weight 240,000 daltons) Absorption Wavelength: 488 nm Emission Wavelength: 575 nm OD566nm/OD280nm: 2.8
Clonality
Polyclonal
Research areas
Secondary antibodies >> anti-Rat >> IgG >> Fluorophore >> Phycoerythrin
Immunology >> Immunoglobulins >> Heavy Chain >> IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab7010 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF
ICC/IF: 1/100 - 1/250.ICC/IF: 1/100 - 1/250.
IHC-P: Use at an assay dependent dilution.
IHC-Fr: Use at an assay dependent dilution.
IM: Use at an assay dependent dilution. (As a general guideline, dilutions of 1/50 to 1/200 should be suitable for most applications.)
Flow Cyt: 1/100 - 1/250.(Use 1 x 106 cells in flow cytometry is approximately 1.0 µg of antibody conjugate.)
References for Goat anti-Rat IgG H&L (PE) secondary antibody (ab7010)
This product has been referenced in:
- Kim SMet al. Acquired resistance to cetuximab is mediated by increased PTEN instability and leads cross-resistance to gefitinib in HCC827 NSCLC cells. Cancer Lett 296:150-9 (2010). Flow Cyt.Read more (PubMed: 20444542) »
See 1 publication for this product
Publishing research using ab7010? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"