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Rat IgG2b, kappa [KLH/G2b-1-2] (FITC) - isotype control (ab128477)

Q&A (2)

Overview

  • Product nameRat IgG2b, kappa [KLH/G2b-1-2] (FITC) - isotype control See all Rat isotype controls ...
  • Description
    Rat IgG2b, kappa [KLH/G2b-1-2] (FITC) - isotype control
  • ConjugationFITC
  • SpecificityThis antibody is specific for KLH and is suitable for use as an isotype control.
  • Tested applicationsFlow Cyt more details

    • Properties

  • FormLiquid
  • Storage instructionsStore at +4°C. Do not freeze.
  • Storage bufferPreservative: 0.1% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • PurityProtein A purified
  • Clonality Monoclonal
  • Clone numberKLH/G2b-1-2
  • IsotypeIgG2b
  • Light chain typekappa
  • Research Areas
    • Alternative names
        Rat Isotype Control

    Applications

    Our Abpromise guarantee covers the use of ab128477 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Flow Cyt Flow Cyt: Use at an assay dependent concentration.

    References for Rat IgG2b, kappa [KLH/G2b-1-2] (FITC) - isotype control (ab128477)

    ab128477 has not yet been referenced specifically in any publications.

    Publishing research using ab128477 ? Please let us know so that we can cite the reference in this datasheet

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    Thank you for your help.
    I have two additional questions:
    - what do you mean as " - no primary - only secondary control" ?
    - can you provide a protocol to use the isotype control you suggest?
    Thank you for your collaboration

    Thank you for your response.

    "No primary control" means that you perform the staining protocol as normal (fixation, blocking, secondary antibody, detection) - except you need to omit the incubation step with the primary antibody. Instea...

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    Inquiry: Dear custumer service, we recently used your HCN4 antibody (ab32675) to perform an immunofluorescence assay on human atrial and ventricular myocytes. Staining was succesful and this data were submitted for publication. One of the reviewer asked t...

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    Thank you for your enquiry and your interest in our products.

    The immunogen used to raise this antibody was a synthetic peptide: SHGSLLLPPA SSPPPPQVPQ RRGTPPLTPG RLTQDLKL, corresponding to amino acids 1048-1085 of Human HCN4:SHGSLLLPPASSPPPPQVPQR...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"