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Rat IgG1, kappa [RTK2071] - isotype control (ab18407)

Q&A (4)

Overview

  • Product nameRat IgG1, kappa [RTK2071] - isotype controlSee all Rat isotype controls ...
  • SpecificityThe RTK2071 immunoglobulin reacts with KLH. The RTK2071 immunoglobulin was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse and human tissues.
  • Tested applicationsIHC-P, IHC-Fr, Flow Cyt, IP, WB more details
  • Immunogen

    Trinitrophenol and KLH.

    • Properties

  • FormLiquid
  • Storage instructionsStore at +4°C.
  • Storage bufferPreservative: None
    Constituents: PBS, pH 7.2
    0.2 µm filter sterilized. Endotoxin level is <0.01 ng/µg protein.
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesAb18407 is low endotoxin and azide free.
  • Clonality Monoclonal
  • Clone numberRTK2071
  • IsotypeIgG1
  • Light chain typekappa
  • Research Areas
    • Alternative names
        Rat Isotype Control

    Applications

    Our Abpromise guarantee covers the use of ab18407 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    IHC-P IHC-P: Use at an assay dependent dilution.
    IHC-Fr IHC-Fr: Use at an assay dependent dilution.
    Flow Cyt Flow Cyt: Use at an assay dependent dilution. For surface or intracellular staining.
    IP IP: Use at an assay dependent dilution.
    WB WB: Use at an assay dependent dilution.

    References for Rat IgG1, kappa [RTK2071] - isotype control (ab18407)

    ab18407 has not yet been referenced specifically in any publications.

    Publishing research using ab18407 ? Please let us know so that we can cite the reference in this datasheet

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    Submit a Question for ab18407

    Displaying 1 - 4 of 4 results for Q&A

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    Votes: Highest First

    Thank you for your help.
    I have two additional questions:
    - what do you mean as " - no primary - only secondary control" ?
    - can you provide a protocol to use the isotype control you suggest?
    Thank you for your collaboration

    Thank you for your response.

    "No primary control" means that you perform the staining protocol as normal (fixation, blocking, secondary antibody, detection) - except you need to omit the incubation step with the primary antibody. Instea...

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    Inquiry: Dear custumer service, we recently used your HCN4 antibody (ab32675) to perform an immunofluorescence assay on human atrial and ventricular myocytes. Staining was succesful and this data were submitted for publication. One of the reviewer asked t...

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    Thank you for your enquiry and your interest in our products.

    The immunogen used to raise this antibody was a synthetic peptide: SHGSLLLPPA SSPPPPQVPQ RRGTPPLTPG RLTQDLKL, corresponding to amino acids 1048-1085 of Human HCN4:SHGSLLLPPASSPPPPQVPQR...

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    Provide the discount code for ab18407 and ab18541

    Expiration date: 25-04-2012
    I am very pleased to hear you would like to use ab18407 and ab18541 in ICC/IF and send us an image of your results.
    This code will give you 1 free [PRIMARY ANTIBODY] before the expiration date. To redeem this offer, p...

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    Hi. I ordered four of your rat isotype control antibodies (18407, 18450, 18536 and 18703). I need to know the isotype of the kappa chains - are they 1a or 1b? I've already asked you once, so please get back to me ASAP. Thanks. Your technical enquiry link ...

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    Thank you for your inquiry. I can confirm that the antibodies are all with kappa light chains as　confirmed with ELISA assay. However, I do not have any information on whether they are 1a or 1b. Regarding the technical enquiry link, as I was able t...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"