Rat IgG1, kappa monoclonal [RTK2071] - Isotype control (ab18412)
Chemical / Small Molecule (Trinitrophenol) conjugated to KLH.
Constituents: PBS, pH 7.2
- Rat Isotype Control
Our Abpromise guarantee covers the use of ab18412 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||IHC-P: Use at an assay dependent dilution.|
|IHC-Fr||IHC-Fr: Use at an assay dependent dilution.|
|Flow Cyt||Flow Cyt: Use 2µg for 106 cells. ab18412 is a negative isotype control and can be used for surface or intracellular staining|
|WB||WB: Use at an assay dependent dilution.|
|IP||IP: Use at an assay dependent dilution.|
Rat IgG1, kappa monoclonal [RTK2071] - Isotype control images
Overlay histogram showing HeLa cells stained with ab16881 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab16881, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rat IgG (H+L) (ab98386) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rat IgG1 [RTK2071] (ab18412, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.
Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
References for Rat IgG1, kappa monoclonal [RTK2071] - Isotype control (ab18412)
ab18412 has not yet been referenced specifically in any publications.