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Rat IgG1, kappa monoclonal [RTK2071] - Isotype control (ab18412)

Q&A (3)

Overview

  • Product nameRat IgG1, kappa monoclonal [RTK2071] - Isotype controlSee all Rat isotype controls ...
  • Tested applicationsIHC-P, IHC-Fr, Flow Cyt, WB, IP more details
  • Immunogen

    Chemical / Small Molecule (Trinitrophenol) conjugated to KLH.

    • Properties

  • FormLiquid
  • Storage instructionsStore at +4°C.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • Isotype control notes The ab18412, RTK2071 immunoglobulin reacts with KLH and was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse and human tissues.
  • Clonality Monoclonal
  • Clone numberRTK2071
  • IsotypeIgG1
  • Light chain typekappa
  • Research Areas
    • Alternative names
        Rat Isotype Control

    Applications

    Our Abpromise guarantee covers the use of ab18412 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    IHC-P IHC-P: Use at an assay dependent dilution.
    IHC-Fr IHC-Fr: Use at an assay dependent dilution.
    Flow Cyt Flow Cyt: Use 2µg for 106 cells. ab18412 is a negative isotype control and can be used for surface or intracellular staining
    WB WB: Use at an assay dependent dilution.
    IP IP: Use at an assay dependent dilution.

    Rat IgG1, kappa monoclonal [RTK2071] - Isotype control images

    • Flow Cytometry - Anti-GTPase HRAS antibody [Y13-259] (ab16881)
      Flow Cytometry - Anti-GTPase HRAS antibody [Y13-259] (ab16881)
      Overlay histogram showing HeLa cells stained with ab16881 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab16881, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rat IgG (H+L) (ab98386) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rat IgG1 [RTK2071] (ab18412, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.

      Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

    References for Rat IgG1, kappa monoclonal [RTK2071] - Isotype control (ab18412)

    ab18412 has not yet been referenced specifically in any publications.

    Publishing research using ab18412 ? Please let us know so that we can cite the reference in this datasheet

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    Customer kindly contacted us regarding the availability of an Igg1 isotype control for ab13789


    Our Rat IgG1, kappa monoclonal Isotype control is a suitable isotype control for ab13789 and has been tested and guaranteed to work in IHC-frozen. A link for this isotype control is given below:
    ab18412 (http://www.abcam.com/Rat-IgG1-kappa-monocl...

    Read More

    MSDS needed for
    ab3104
    ab18412
    ab45139
    ab68672

    Please find attached to this email the MSDS you requested for the following antibodies:
    ab3104
    ab18412
    ab45139
    ab68672

    A link to each of those MSDS will be shortly available from the product datasheets. This usually takes a few ho...

    Read More

    Dear,

    I put in this email all the reference for which I need a MSDS.

    ab45139
    ab3104
    ab68672
    ab45139
    ab18412

    Thanks in advance for your answer,

    Thank you for contacting us.

    I would like to inform you that we are currently dealing with your request.

    I will contact you again as soon as the requested documents are available.

    Thank you for your patience.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"