Overview
Isotype Control monoclonal antibodies are used to estimate the non-specific binding of target primary antibodies to cell surface antigens. Non-specific binding is due to Fc receptor binding or other protein-protein interactions. Isotype controls show negligible cross-reactivity with cell surface antigens on tissue sections or in cellular preparations. Isotype Controls should be used at identical concentrations and staining conditions as the target primary antibodies.
Properties
Constituents: PBS, pH 7.2
Concentration information loading...-
Alternative names
- Rat Isotype Control
Applications
Our Abpromise guarantee covers the use of ab35774 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| Flow Cyt | Flow Cyt: Use at an assay dependent dilution. |
| WB | WB: Use at an assay dependent dilution. |
| IP | IP: Use at an assay dependent dilution. |
| IHC-Fr | IHC-Fr: Use at an assay dependent dilution. |
| IHC-P | IHC-P: Use at an assay dependent dilution. |
References for Rat IgM, kappa monoclonal [RTK2118] (FITC) - Isotype control (ab35774)
ab35774 has not yet been referenced specifically in any publications.