Overview

  • Product name
    Rat L-FABP ELISA Kit
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall < 10%
    Inter-assay
    Sample n Mean SD CV%
    Overall < 10%
  • Sample type
    Urine, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    1.278 ng/ml
  • Range
    3.125 ng/ml - 200 ng/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum > 85 % - %

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Rat
  • Product overview

    Abcam’s L-FABP Rat ELISA Kit (ab190545) is an in vitro enzyme-linked immunosorbent assay (ELISA) for the quantitative measurement of L-FABP in rat serum and plasma.

    In this assay the L-FABP present in samples reacts with the anti-L-FABP antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-L-FABP antibodies conjugated with horseradish peroxidase (HRP) are added. These enzyme-labeled antibodies form complexes with the previously bound L-FABP. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of L-FABP in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of L-FABP in the test sample. The quantity of L-FABP in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

  • Function
    Binds free fatty acids and their coenzyme A derivatives, bilirubin, and some other small molecules in the cytoplasm. May be involved in intracellular lipid transport.
  • Sequence similarities
    Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.
  • Domain
    Forms a beta-barrel structure that accommodates hydrophobic ligands in its interior.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Alternative names
    • fabp1
    • FABPL
    • FABPL_HUMAN
    • Fatty acid binding protein 1, liver
    • Fatty acid binding protein, liver
    • Fatty acid-binding protein
    • Fatty acid-binding protein 1
    • L-FABP
    • liver
    • Liver-type fatty acid-binding protein
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab190545 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Rat L-FABP ELISA Kit images

  • Representative standard curve using ab190545 L-FABP Rat ELISA Kit.

Protocols

References for Rat L-FABP ELISA Kit (ab190545)

ab190545 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab190545.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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