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|Sample type||Average %||Range|
|Serum||> 85||% - %|
Abcam’s L-FABP Rat ELISA Kit (ab190545) is an in vitro enzyme-linked immunosorbent assay (ELISA) for the quantitative measurement of L-FABP in rat serum and plasma.
In this assay the L-FABP present in samples reacts with the anti-L-FABP antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-L-FABP antibodies conjugated with horseradish peroxidase (HRP) are added. These enzyme-labeled antibodies form complexes with the previously bound L-FABP. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of L-FABP in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of L-FABP in the test sample. The quantity of L-FABP in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
|Components||1 x 96 tests|
|20X Wash Buffer Concentrate||1 x 50ml|
|L-FABP Rat 1X Diluent||1 x 60ml|
|L-FABP Rat Antibody coated microwells||1 x 96 tests|
|L-FABP Rat Calibrator (Lyophilized)||1 vial|
|L-FABP Rat HRP Conjugate||1 vial|
|Stop Solution||1 x 12ml|
|TMB Substrate||1 x 12ml|
Our Abpromise guarantee covers the use of ab190545 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab190545 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"