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Rat monoclonal [SB84a] Secondary Antibody to Mouse IgG2a - gamma chain (ab79091)

Submit an Abreview Q&A (6)

Overview

  • Product nameRat monoclonal [SB84a] Secondary Antibody to Mouse IgG2a - gamma chainSee all IgG2a secondary antibodies ...
  • Target speciesMouse
  • SpecificityReacts with the heavy chain of IgG2a.
  • Tested applicationsFlow Cyt, ELISA, ICC/IF more details
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • Positive controlHuman PBMC

Properties

Applications

Our Abpromise guarantee covers the use of ab79091 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Flow Cyt
ELISA
ICC/IF
  • Application notesELISA: Use at an assay dependent dilution.
    Flow Cyt: Use at an assay dependent dilution.
    ICC/IF: Use at an assay dependent dilution.
    Second step reagent for mouse IgG2a monoclonal antibodies

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • References for Rat monoclonal [SB84a] Secondary Antibody to Mouse IgG2a - gamma chain (ab79091)

    ab79091 has not yet been referenced specifically in any publications.

    Publishing research using ab79091 ? Please let us know so that we can cite the reference in this datasheet

    Product Wall

    Submit an Abreview for ab79091 Submit a Question for ab79091

    Displaying 1 - 6 of 6 results for Abreviews and Q&A

    10
    Votes: Highest First

    Regarding the antibodies we use(ab13418, ab79091), what is the cataloque number of the anti-rat-Ig G-HRP you offer for us as a tertiary antibody.

    Thank you again.

    Thank you for contacting us.

    Please buy ab7097.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    I have another question: as you sad before ab79091 is an unconjugated secondary antibody; what should be the next step before DAB (we use DAB plus substrate, Lab vision, TA-125-HDX in our laboratory) in our protocol?

    Looking forward to hearing fr...

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    Thank you for contacting us.

    Once the incubation with secondary antibody is finished then you need to add tertiary antibody which is in your case would be anti rat IgG-HRP. Please incubate for 1 hour at room temperature. Please make sure the tert...

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    Thank you for your help.
    My next question was about the antigen retrieval step:
    Do you offer an enzymatic retrieval with tripsin enzyme for my protocol.
    Because the HIER destructs my sections. This is another big problem for me.

    Thank you for contacting us.

    We have a Trypsin Enzymatic Antigen Retrieval Solution in our catalogue with reference ab970 (http://www.abcam.com/ab970).

    I hope this information is helpful to you. Please do not hesitate to contact us i...

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    Dear Abcam Technical Support,

    A customer has contacted us with a technical enquiry regarding the following antibodies:

    http://www.abcam.com/mcherry-antibody-1c51-ab125096.html (Anti-mCherry antibody [1C51]) and http://www.abcam.com/gfp-...

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    Thank you for contacting us.

    1. To our knowledge the ab125096 has not been tested in IHC-Fr yet and we can not guarantee the product to detect the mCherry in IHC-Fr. Regrettably do not offer any other mCherry antibody. However, if the customer li...

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    Thank you for dealing with our problem: I will explain our protocol step by step:
    Protocol
    1. Formalin fixed (24-48 hr) parafin embedded blocks (decalcified with EDTA) & 4 Mm thick sections on adhesive slides.
    2. Deparafinizati...

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    The protocol seems fine to me. However the following updates can be tried

    - 0.3% H2O2 can be incubated for 10-15 minutes only.
    - The endogenous peroxidases can be blocked after addition of primary antibody. Please check whether the tissue sec...

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    Product code: 79091
    Lot number: GR68157-1
    Inquiry: Hi, I have written you before about the staining problem with ab13418 and ab79091 products. I want to ask one more question about the Peroxidase step after the secondary antibody step. Is it neces...

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    Thank you for contacting us.

    I have asked few questions in my previous email so please send be answers asap. It would be very helpful if you could send me your step by step protocol as I suspect the protocol might needs updates.

    Looking ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"