Rb antibody [Rb1 1F8] - Nuclear Marker (ab24)

Product Name 

Rb antibody [Rb1 1F8] - Nuclear Marker

Product type 

Primary antibodies

Description 

Mouse monoclonal [Rb1 1F8] to Rb - Nuclear Marker

Immunogen 

Rb-b-galactosidase fusion protein spanning nucleotides 1126-1973 of human Rb gel purified from bacterial lysates (R. Grand et al. 1989).
BLAST 'IMMCSMYG I CKVKNIDLK FKIIVTAYKD LPHAVQETF K RVLIKEEE YD SIIVFYN SVF MQRLKT ' with or

Reacts with 

Hu

Predicted to react 
(species key)

Ms, Chk, Chmp
(Due to sequence homology)

Specificity 

This antibody reacts with hyperphosphorylated and un (under) phosphorylated forms of Rb protein.

Epitope 

The epitope has been mapped between aa 703-772 of human RB1.

Tested applications 
(see key)

IHC-Fr, IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
IHC-Fr: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Predicted molecular weight: 105 kDa.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Tested in a panel of human cell lines and CV-1 cell line (established from monkey kidney epithelium).

Cellular localization 

Nuclear

Research areas 

Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> Rb family

Chromatin and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Tumor Suppressors
Tags & Cell Markers >> Subcellular Markers >> Nucleus >> Nucleoplasm
Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> Rb

Relevance 

Rb is a tumor suppressor gene which functions as a negative regulator of the cell cycle by interacting with transcription factors including E2F1, PU1, ATF2, UBF, Elf1 and cAbl. This ability of Rb to alter transcription is regulated by phosphorylation catalyzed by the cyclin dependent protein kinases (cdks). Rb is phosphorylated on serine and threonine, but not on tyrosine residues. It forms a complex with SV40 large T antigen, adenovirus E1A, and human papilloma virus 16E. Rb protein may act by regulating transcription and loss of its function leads to uncontrolled cell growth. Aberrations in the Rb gene have been implicated in cancers of breast, colon, prostate, kidney, nasopharynx, and leukemia.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

Rb1 1F8

Myeloma 

Sp2/0-Ag14

Isotype 

IgG1

Purity 

Protein A purified

Storage buffer 

Preservative: None
Constituents: PBS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

See below for Rb antibody [Rb1 1F8] - Nuclear Marker images, references, products related to ab24 and other tools.

Rb antibody [Rb1 1F8] - Nuclear Marker images:

References for Rb antibody [Rb1 1F8] - Nuclear Marker (ab24)

  This product has been used in: (two most recent references)

Ozeki M & Shively JE Differential cell fates induced by all-trans retinoic acid-treated HL-60 human leukemia cells. J Leukoc Biol : (2008). PubMed: 18552205

Ying L et al. Chronic inflammation promotes retinoblastoma protein hyperphosphorylation and E2F1 activation. Cancer Res 65:9132-6 (2005). WB; Mouse. PubMed: 16230367

See all 5 publication references for this product.


If you publish research using ab24 please let us know so that we can cite the reference on this datasheet.

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