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Western blot - Rb antibody [Rb1 1F8] - Nuclear Marker (ab24)
![Western blot - Rb antibody [Rb1 1F8] - Nuclear Marker (ab24)](/ps/datasheet/images/ab24_2.jpg)
(enlarge)
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Product Name
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Rb antibody [Rb1 1F8] - Nuclear Marker
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See all Rb antibodies (40)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [Rb1 1F8] to Rb - Nuclear Marker
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Immunogen
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Rb-b-galactosidase fusion protein spanning nucleotides 1126-1973 of human Rb gel purified from bacterial lysates (R. Grand et al. 1989). BLAST 'IMMCSMYG I CKVKNIDLK FKIIVTAYKD LPHAVQETF K RVLIKEEE YD SIIVFYN SVF MQRLKT ' with or 
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Reacts with
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Hu
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Predicted to react
(species key)
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Ms, Chk, Chmp (Due to sequence homology)
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Specificity
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This antibody reacts with hyperphosphorylated and un (under) phosphorylated forms of Rb protein.
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Epitope
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The epitope has been mapped between aa 703-772 of human RB1.
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Tested applications
(see key)
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IHC-Fr, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions IHC-Fr: Use at an assay dependent dilution. IP: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Predicted molecular weight: 105 kDa.
Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Tested in a panel of human cell lines and CV-1 cell line (established from monkey kidney epithelium).
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Cellular localization
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Nuclear
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Research areas
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Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> Rb family
Chromatin and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Tumor Suppressors Tags & Cell Markers >> Subcellular Markers >> Nucleus >> Nucleoplasm Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> Rb
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Relevance
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Rb is a tumor suppressor gene which functions as a negative regulator of the cell cycle by interacting with transcription factors including E2F1, PU1, ATF2, UBF, Elf1 and cAbl. This ability of Rb to alter transcription is regulated by phosphorylation catalyzed by the cyclin dependent protein kinases (cdks). Rb is phosphorylated on serine and threonine, but not on tyrosine residues. It forms a complex with SV40 large T antigen, adenovirus E1A, and human papilloma virus 16E. Rb protein may act by regulating transcription and loss of its function leads to uncontrolled cell growth. Aberrations in the Rb gene have been implicated in cancers of breast, colon, prostate, kidney, nasopharynx, and leukemia.
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Database links
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The links below go to external sites and will open in a new browser window
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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Rb1 1F8
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Myeloma
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Sp2/0-Ag14
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Isotype
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IgG1
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Purity
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Protein A purified
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Storage buffer
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Preservative: None Constituents: PBS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Rb antibody [Rb1 1F8] - Nuclear Marker is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Rb antibody [Rb1 1F8] - Nuclear Marker images, references, products related to ab24 and other tools.
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Rb antibody [Rb1 1F8] - Nuclear Marker images:
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Western blot - Rb antibody [Rb1 1F8] - Nuclear Marker (ab24)
Rb antibody [Rb1 1F8] - Nuclear Marker (ab24) at 1/1000 dilution + Human Fibroblast cell lysate(nuclear). 16 hour incubation.
Secondary NIF 825 (provided in ECL kit) - HRP conjugated developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 105 kDa Observed band size : 100-150 kDa (why is the actual band size different from the predicted?)
Exposure time : 1 minute
This image is courtesy of an Abreview submitted on 5 October 2005.
See Abreview
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References for Rb antibody [Rb1 1F8] - Nuclear Marker (ab24)
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This product has been used in: (two most recent references)
Ozeki M & Shively JE Differential cell fates induced by all-trans retinoic acid-treated HL-60 human leukemia cells. J Leukoc Biol : (2008). PubMed: 18552205
Ying L et al. Chronic inflammation promotes retinoblastoma protein hyperphosphorylation and E2F1 activation. Cancer Res 65:9132-6 (2005). WB; Mouse. PubMed: 16230367
See all 5 publication references for this product.
If you publish research using ab24 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to Rb
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Rb antibody [Rb1 1F8] - Nuclear Marker - more information
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Rb antibody [Rb1 1F8] - Nuclear Marker - related products:
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Rb antibody [Rb1 1F8] - Nuclear Marker - other tools:
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Contact Abcam with a Technical Enquiry about Rb antibody [Rb1 1F8] - Nuclear Marker |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"