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All lanes : Anti-Rb (phospho S780) antibody [E182] (ab32513) at 1/1000 dilution
Lane 1 : Untreated Jurkat cell lysate
Lane 2 : Jurkat cell lysate treated with FBS
Lane 3 : Jurkat cell lysate treated with Alkaline Phosphatase.
Predicted band size : 106 kDa
Observed band size : 110 kDa (why is the actual band size different from the predicted?)
Immunofluorescent staining of (A) non-treated and (B) Lambda Phosphatase-treated A431 cells using anti-Phospho-Rb (S780) ab32513 Rabbit Monoclonal Antibody
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