Products:Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> Rb
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Hi there, I was wondering if you could let me know if you have any pure positive controls or blocking peptides for either of these two antibodies: Anti-Phospho Rb (T821) #ab4787 Anti-Rb (ab6075) For both there are suggested cell lysates to use as positive controls, but I need a pure protein to verify the exact band on my western blots. I know you provide a His-tagged version of the Rb full length protein, but do you have any non-tagged versions? Or do you know a good vendor for these? Also, the blocking peptide for phospho-Rb is no longer available (#ab5324); is there another source I could use to get this blocking peptide? |
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ANSWER: |
Thank you for contacting us. We currently only have the tagged Rb proteins in our catalog and no blocking peptide for ab4787 is available. I would sugges Biocompare.com to search for these products. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information. |
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Predicted band size : 106 kDa
Peptide Competition: Cell extracts prepared from Jurkat cells in high growth phase were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF. Membranes were incubated with 0.50 µg/mL ab4787, following prior incubation in the: absence of the phosphopeptide immunogen (1), a generic phosphothreonine containing peptide (2), the non phosphopeptide corresponding to the phosphopeptide immunogen (3), or the presence of the phosphopeptide immunogen (4). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase and bands were detected using the Tropix WesternStar detection method. The data show that only the phosphopeptide corresponding to this site blocks the antibody signal, demonstrating the specificity of the ab4787 antibody for this epitope.
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