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Western blot - Rb (phospho T821) antibody (ab4787)
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Product Name
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Rb (phospho T821) antibody
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See all Rb antibodies (40)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Rb (phospho T821)
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Immunogen
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Synthetic peptide (Human) derived from a region of human Rb that contains threonine 821 (based on Swiss Protein database, accession number P06400). The sequence is conserved in human, mouse and rat.
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Reacts with
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Hu
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Predicted to react
(species key)
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Ms, Rat
(Due to sequence homology)
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: 1/1000. Detects a band of approximately 120 kDa (predicted molecular weight: 106 kDa). Can be blocked with Rb peptide - phospho T821 (phospho and non-phospho pair).
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Jurkat cells in high growth phase.
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Cellular localization
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Nuclear
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Research areas
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Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> Rb family
Chromatin and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Tumor Suppressors
Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> Rb
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Relevance
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Rb is a tumor suppressor gene which functions as a negative regulator of the cell cycle by interacting with transcription factors including E2F1, PU1, ATF2, UBF, Elf1 and cAbl. This ability of Rb to alter transcription is regulated by phosphorylation catalyzed by the cyclin dependent protein kinases (cdks). Rb is phosphorylated on serine and threonine, but not on tyrosine residues. It forms a complex with SV40 large T antigen, adenovirus E1A, and human papilloma virus 16E. Rb protein may act by regulating transcription and loss of its function leads to uncontrolled cell growth. Aberrations in the Rb gene have been implicated in cancers of breast, colon, prostate, kidney, nasopharynx, and leukemia.
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Rb (phospho T821) antibody (ab4787) |
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol, PBS, 1mg/ml BSA. pH 7.3 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Purification notes
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Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated Rb protein. The final product is generated by affinity chromatography using a Rb-derived peptide that is phosphorylated at threonine 821.
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Form
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Liquid
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Rb (phospho T821) antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Rb (phospho T821) antibody images, references, products related to ab4787 and other tools.
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Rb (phospho T821) antibody images:
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 Western blot - Rb (phospho T821) antibody (ab4787)
Predicted band size : 106 kDa
Peptide Competition: Cell extracts prepared from Jurkat cells in high growth phase were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF. Membranes were incubated with 0.50 µg/mL ab4787, following prior incubation in the: absence of the phosphopeptide immunogen (1), a generic phosphothreonine containing peptide (2), the non phosphopeptide corresponding to the phosphopeptide immunogen (3), or the presence of the phosphopeptide immunogen (4). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase and bands were detected using the Tropix WesternStar detection method. The data show that only the phosphopeptide corresponding to this site blocks the antibody signal, demonstrating the specificity of the ab4787 antibody for this epitope.
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References for Rb (phospho T821) antibody (ab4787)
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This product has been used in: (two most recent references)
Labalette C et al. The LIM-only Protein FHL2 Regulates Cyclin D1 Expression and Cell Proliferation. J Biol Chem 283:15201-15208 (2008). WB; Mouse. PubMed: 18378678
Nakagawa M et al. Phorbol erster induced G1 phase arrest selectively mediated by a PKCdelta -dependent induction of p21CIP1. J Biol Chem : (2005). PubMed: 16055435
See all 6 publication references for this product.
If you publish research using ab4787 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to Rb
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Rb (phospho T821) antibody - more information
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