![Western blot - RbAp48 antibody [11G10] (ab488) image](#ab488-11996_copy.jpg)
![Western blot - RbAp48 antibody [11G10] (ab488) image](#RbAp48-Primary-antibodies-ab488-1.jpg)
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Overview
Product name
Anti-RbAp48 antibody [11G10]
See all RbAp48 products (14) ...
Description
Mouse monoclonal [11G10] to RbAp48
Tested applications
WB, IP, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Fusion protein containing the complete coding region (amino acids 1-425) of RbAp48 expressed in E. coli.
Positive control
Molt 4, HeLa cells.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 10mM PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Protein G purified
Purification notes
Purified from ascites by protein G chromatography to at least 95% homogeneity as determined by SDS-PAGE.
Primary antibody notes
This protein is a ubiquitously expressed nuclear protein and belongs to a highly conserved subfamily of WD-repeat proteins. It is found among several proteins that binds directly to retinoblastoma protein, which regulates cell proliferation. The encoded protein is found in many histone deacetylase complexes, including mSin3 co-repressor complex. It is also present in protein complexes involved in chromatin assembly. This protein can interact with BRCA1 tumor-suppressor gene and may have a role in the regulation of cell proliferation and differentiation.
Clonality
Monoclonal
Clone number
11G10
Myeloma
NS1
Isotype
IgG2b
Light chain type
kappa
Research areas
Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> Rb family
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation >> HDACs >> Class I
Epigenetics and Nuclear Signaling >> Cell cycle >> Cell Cycle Inhibitors >> Rb
Epigenetics and Nuclear Signaling >> Chromatin Remodeling >> Polycomb Silencing >> PRC2
Epigenetics and Nuclear Signaling >> Chromatin Remodeling >> ISWI/NURF/CHRAC
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation
Applications
Show applications key
Our Abpromise guarantee covers the use of ab488 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 - 5 µg/ml.Predicted molecular weight: 47.5 kDa.(Use at a concentration of 1 - 5 µg/ml. Predicted molecular weight: 47.5 kDa. Dilute in 5% Milk/PBST and incubate at 4ºC overnight. Use under reducing conditions. Can be blocked with RbAp 48 peptide)
IP: Use a concentration of 1 - 5 µg/ml.
ICC/IF: 1/500 - 1/1000.(1/500 - 1/1000. Permeabilization step recommended. Use in PBST containing 2%BSA and 10% normal serum at 4ºC overnight.)
Target
Function
Core histone-binding subunit that may target chromatin assembly factors, chromatin remodeling factors and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. Component of several complexes which regulate chromatin metabolism. These include the chromatin assembly factor 1 (CAF-1) complex, which is required for chromatin assembly following DNA replication and DNA repair; the core histone deacetylase (HDAC) complex, which promotes histone deacetylation and consequent transcriptional repression; the nucleosome remodeling and histone deacetylase complex (the NuRD complex), which promotes transcriptional repression by histone deacetylation and nucleosome remodeling; the PRC2/EED-EZH2 complex, which promotes repression of homeotic genes during development; and the NURF (nucleosome remodeling factor) complex.
Sequence similarities
Belongs to the WD repeat RBAP46/RBAP48/MSI1 family.
Contains 6 WD repeats.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ09028
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CAF 1 subunit C antibody
- CAF I 48 kDa subunit antibody
- CAF I p48 antibody
see all
Database links
- Entrez Gene: 5928 Human
- Entrez Gene: 19646 Mouse
- Omim: 602923 Human
- SwissProt: Q09028 Human
- SwissProt: Q60972 Mouse
- Unigene: 16003 Human
- Unigene: 12145 Mouse
Anti-RbAp48 antibody [11G10] images:
Western blot - RbAp48 antibody [11G10] (ab488)
![Western blot - RbAp48 antibody [11G10] (ab488)](/ps/datasheet/Images/0/ab488/ab488-11996_copy.jpg)
Anti-RbAp48 antibody [11G10] (ab488) at 2 µg/ml + Whole cell lysates prepared from human colon adenocarcinoma SW480 cell line at 30 µg
Secondary
HRP-conjugated goat anti-mouse IgG at 1/10000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 47.5 kDa
Exposure time : 10 minutes
This image is courtesy of an anonymous Abreview
Western blot - RbAp48 antibody [11G10] (ab488)
![Western blot - RbAp48 antibody [11G10] (ab488)](/ps/datasheet/images/0/ab488/RbAp48-Primary-antibodies-ab488-1.jpg)
Predicted band size : 47.5 kDa
ab488 used in Western Blot of nuclear extract prepared from female mouse ES cells.Western blot assays using antibodies specific for the PTB, Suz12, RbAp46, RbAp48 (ab488), Eed, and Ezh2 protein were used to evaluate the relative amounts of these proteins in the purified complexes formed with the various RNAs.
Image from S Maenner et al, PLoS Biol 8:e1000276 (2010), Fig 9.
References for Anti-RbAp48 antibody [11G10] (ab488)
This product has been referenced in:
- Maenner Set al. 2-D structure of the A region of Xist RNA and its implication for PRC2 association. PLoS Biol 8:e1000276 (2010). WB.Read more (PubMed: 20052282) »
- Van Dessel Net al. The phosphatase interactor NIPP1 regulates the occupancy of the histone methyltransferase EZH2 at Polycomb targets. Nucleic Acids Res 38:7500-12 (2010). WB; Human.Read more (PubMed: 20671031) »
See all 5 publications for this product
Publishing research using ab488? Please let us know so that we can cite the reference in this datasheet
![RbAp48 antibody [11G10] for WB in Human (488)](/ps/Reviews/Images/ab10618_55043.jpg)
![RbAp48 antibody [11G10] for WB in Human (488)](/ps/Reviews/Images/ab11996_64368.jpg)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"