Anti-RbAp48 antibody [2D7] (ab117746)

Overview

• Product nameAnti-RbAp48 antibody [2D7]See all RbAp48 primary antibodies ...
• Description
  Mouse monoclonal [2D7] to RbAp48
• Tested applicationsWB, IHC-P, ICC/IF, ELISA more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Recombinant protein of Human RbAp48.

• Positive controlHeLa cells and lysate, Human Testis tissue.

Properties

• FormLiquid
• Storage instructionsStore at +4°C or -20°C long term. Avoid repeated freeze / thaw cycles. Store undiluted.
• Storage bufferpH: 7.20
  Constituent: 99% PBS
• Concentration information loading...
• PurityProtein A purified
• Clonality Monoclonal
• Clone number2D7
• IsotypeIgG1
• Light chain typekappa
• Research Areas
  • Cancer
  • Oncoproteins/suppressors
  • Tumor suppressors
  • Rb family

  • Epigenetics and Nuclear Signaling
  • Chromatin Remodeling
  • ISWI/NURF/CHRAC

  • Epigenetics and Nuclear Signaling
  • Cell cycle
  • Cell Cycle Inhibitors
  • Rb

  • Epigenetics and Nuclear Signaling
  • Chromatin Remodeling
  • Polycomb Silencing
  • PRC2

  • Epigenetics and Nuclear Signaling
  • Chromatin Modifying Enzymes
  • Acetylation
  • HDACs
  • Class I

Applications

Target

• FunctionCore histone-binding subunit that may target chromatin assembly factors, chromatin remodeling factors and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. Component of several complexes which regulate chromatin metabolism. These include the chromatin assembly factor 1 (CAF-1) complex, which is required for chromatin assembly following DNA replication and DNA repair; the core histone deacetylase (HDAC) complex, which promotes histone deacetylation and consequent transcriptional repression; the nucleosome remodeling and histone deacetylase complex (the NuRD complex), which promotes transcriptional repression by histone deacetylation and nucleosome remodeling; the PRC2/EED-EZH2 complex, which promotes repression of homeotic genes during development; and the NURF (nucleosome remodeling factor) complex.
• Sequence similaritiesBelongs to the WD repeat RBAP46/RBAP48/MSI1 family.
  Contains 6 WD repeats.
• Cellular localizationNucleus.

Target information above from: UniProt accession Q09028 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 5928 Human
  • Omim: 602923 Human
  • SwissProt: Q09028 Human
  • Unigene: 16003 Human

• Alternative names
  CAF I p48 antibodyCAF-1 subunit C antibodyCAF-I 48 kDa subunit antibody

  CAF-I p48 antibodyChromatin assembly factor 1 subunit C antibodyChromatin assembly factor I p48 subunit antibodyChromatin assembly factor/CAF 1 p48 subunit antibodyHistone-binding protein RBBP4 antibodyMSI1 protein homolog antibodyNucleosome-remodeling factor subunit RBAP48 antibodyNURF55 antibodyRbAp 48 antibodyRBAP48 antibodyRBBP-4 antibodyRBBP4 antibodyRBBP4_HUMAN antibodyRetinoblastoma binding protein 4 antibodyRetinoblastoma binding protein p48 antibodyRetinoblastoma-binding protein 4 antibodyRetinoblastoma-binding protein p48 antibody

  see all

Anti-RbAp48 antibody [2D7] images

• 

  Western blot - Anti-RbAp48 antibody [2D7] (ab117746)
  Anti-RbAp48 antibody [2D7] (ab117746) + HeLa cell lysate at 500 µg/ml
  
  Predicted band size : 48 kDa
  
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RbAp48 antibody [2D7] (ab117746)
  ab117746 at a concentration of 5 µg/ml staining RbAp48 in Formalin-Fixed, Paraffin-Embedded Human Testis.
• 

  Immunocytochemistry/ Immunofluorescence - Anti-RbAp48 antibody [2D7] (ab117746)
  Immunofluorescence of ab117746 on HeLa cells. [antibody concentration 10 ug/ml]
• 

  Eli-pair - Anti-RbAp48 antibody [2D7] (ab117746)
  Detection limit for RbAp48 is approximately 0.3ng/ml as a capture antibody.