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If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab1765? Please let us know so that we can cite the reference in this datasheet
ab1765 has been referenced in 2 publications.
- Pegoraro G et al. Ageing-related chromatin defects through loss of the NURD complex. Nat Cell Biol 11:1261-7 (2009). ICC/IF; Human. PubMed: 19734887
- Nitzsche A et al. Temporal Dynamics of Cytomegalovirus Chromatin Assembly in Productively Infected Human Cells. J Virol : (2008). WB, ICC/IF; Human. PubMed: 18786996
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - p48 CAF1 antibody (ab1765)
Predicted band size : 47.6 kDa
Western blot - p48 CAF1 antibody (ab1765)
Anti-RbAp48 antibody (ab1765) at 1/1000 dilution
Secondary
Anti-rabbit IgG HRP
developed using the ECL technique
Predicted band size : 47.6 kDa
Observed band size : 40,55 kDa (why is the actual band size different from the predicted?)
Exposure time : 1 minute
ab1765 detecting p48 CAF1 from human LCL whole cell lysates by Western Blot.
Immunoprecipitation - p48 CAF1 antibody (ab1765)
ab1765 used in Immunoprecipitation of HeLa whole cell lysate.
Whole cell extract (containing 100µg of soluble protein) was incubated with 20µg of antibody overnight. Protein A-Agarose was added to the immunocomplexes and incubated at room temperature for three hours. Immunocomplexes were eluted and then resolved using 7.5% SDS-PAGE under reducing conditions. For the detection of HDAC1, 2 and 3 a non-Abcam mouse monoclonal antibody was used at a dilution of 1/800. Results support the understanding that HDAC1 and 2 are in the same complex together with p48. Note that HDAC1 does not coprecipitate HDAC2, probably displaced by the antibody during the experiment.
Lane 1: Input
Lane 2: HDAC1 (ab1769)
Lane 3: p48 (ab1765)
Lane 4: No antibody control
This image is courtesy of an Abreview by Hugh Spotswood submitted on 9 February 2006.
Immunocytochemistry/ Immunofluorescence - p48 CAF1 antibody (ab1765)
ab1765 staining cultured human HeLa cells by ICC/IF. Cells were PFA fixed and permeabilized in 0.5% Triton X100 prior to blocking in 5% BSA for 1 hour at 20°C. The primary antibody was diluted 1/50 and incubated with the sample for 1 hour at 20°C. A Cy3® conjugated donkey anti-rabbit antibody was used as the secondary.
This image is courtesy of an Abreview submitted by Dr Alexander Rapp
p48 CAF1 antibody - ChIP Grade for WB in Mouse (1765)
p48 CAF1 antibody for IP in Human (1765)
p48 CAF1 antibody - ChIP Grade for in Human (1765)
p48 CAF1 antibody for WB in Human (1765)
p48 CAF1 antibody for ICC/IF in Human (1765)
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