Overview

  • Product nameAnti-RbAp48 antibodySee all RbAp48 primary antibodies ...
  • Description
    Rabbit polyclonal to RbAp48
  • Tested applicationsICC/IF, WB more details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Chicken, Cow, Xenopus laevis, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to Human RbAp48 aa 1-14.
    Sequence: MADKFAAFDDAVEEC
    (Peptide available as ab5014)

  • Positive control
    • WB: HeLa cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab3536 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ICC/IF Use at an assay dependent concentration.
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 47.5 kDa).Can be blocked with RbAp48 peptide (ab5014).

A larger ~60 kDa unknown protein from HeLa cell lysate is also detected.

Target

  • FunctionCore histone-binding subunit that may target chromatin assembly factors, chromatin remodeling factors and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. Component of several complexes which regulate chromatin metabolism. These include the chromatin assembly factor 1 (CAF-1) complex, which is required for chromatin assembly following DNA replication and DNA repair; the core histone deacetylase (HDAC) complex, which promotes histone deacetylation and consequent transcriptional repression; the nucleosome remodeling and histone deacetylase complex (the NuRD complex), which promotes transcriptional repression by histone deacetylation and nucleosome remodeling; the PRC2/EED-EZH2 complex, which promotes repression of homeotic genes during development; and the NURF (nucleosome remodeling factor) complex.
  • Sequence similaritiesBelongs to the WD repeat RBAP46/RBAP48/MSI1 family.
    Contains 6 WD repeats.
  • Cellular localizationNucleus.
  • Target information above from: UniProt accession Q09028 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • CAF 1 subunit C antibody
    • CAF I 48 kDa subunit antibody
    • CAF I p48 antibody
    • CAF-1 subunit C antibody
    • CAF-I 48 kDa subunit antibody
    • CAF-I p48 antibody
    • Chromatin assembly factor 1 subunit C antibody
    • Chromatin assembly factor I p48 subunit antibody
    • Chromatin assembly factor/CAF 1 p48 subunit antibody
    • Histone binding protein RBBP4 antibody
    • Histone-binding protein RBBP4 antibody
    • MSI1 protein homolog antibody
    • Nucleosome remodeling factor subunit RBAP48 antibody
    • Nucleosome-remodeling factor subunit RBAP48 antibody
    • NURF 55 antibody
    • NURF55 antibody
    • RbAp 48 antibody
    • RBAP48 antibody
    • RBBP 4 antibody
    • RBBP-4 antibody
    • RBBP4 antibody
    • RBBP4_HUMAN antibody
    • Retinoblastoma binding protein 4 antibody
    • Retinoblastoma binding protein p48 antibody
    • Retinoblastoma-binding protein 4 antibody
    • Retinoblastoma-binding protein p48 antibody
    see all

Anti-RbAp48 antibody images

  • ab3536 staining RbAp48 in Human HeLa cells by immunocytochemistry/ Immunofluorescence.



  • Predicted band size : 47.5 kDa

    Western blot of RpAp48 on 1) Du-148 2) LNCaP and 3) Hep-G2 cell extracts using ab3536.

References for Anti-RbAp48 antibody (ab3536)

This product has been referenced in:
  • Sridharan R & Smale ST Predominant interaction of both Ikaros and Helios with the NuRD complex in immature thymocytes. J Biol Chem 282:30227-38 (2007). WB ; Human . Read more (PubMed: 17681952) »

See 1 Publication for this product

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