Anti-RbAp48 antibody (ab3536)

Overview

• Product nameAnti-RbAp48 antibodySee all RbAp48 primary antibodies ...
• Description
  Rabbit polyclonal to RbAp48
• Tested applicationsWB more details
• Species reactivity
  Reacts with: Human
  Predicted to work with: Mouse, Chicken, Xenopus laevis, Zebrafish
• Immunogen

  Synthetic peptide: MADKFAAFDDAVEEC, corresponding to amino acids 1-14 of Human RbAp 48.

  Run BLAST with Run BLAST with

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPBS, 1 mg/ml BSA, 0.05% sodium azide
• Concentration information loading...
• PurityImmunogen affinity purified
• Primary antibody notes Retinoblastoma protein (Rb) associated protein 48 (RbAp 48) is a nuclear protein that has been shown to interact directly with retinoblastoma protein and is a component of the mSin3 corepressor complex as are the histone deacetylase proteins (HDACs). RbAp 48 shares about 75% sequence identity with the closely related RbAp 46, and appears to be functionally related to the yeast MSI 1 protein in that overexpression of RbAp 48 suppresses heat shock sensitivity in a RAS pathway dependent manner. RbAp48 has also been demonstrated to be up-regulated by the Wilms’ tumor suppressor gene product, WT1. Additionally, cells transfected with RbAp 48 have shown strong inhibition of growth.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Cancer
  • Oncoproteins/suppressors
  • Tumor suppressors
  • Rb family

  • Epigenetics and Nuclear Signaling
  • Chromatin Remodeling
  • Polycomb Silencing
  • PRC2

  • Epigenetics and Nuclear Signaling
  • Chromatin Modifying Enzymes
  • Acetylation
  • HDACs
  • Class I

  • Epigenetics and Nuclear Signaling
  • Cell cycle
  • Cell Cycle Inhibitors
  • Rb

  • Epigenetics and Nuclear Signaling
  • Chromatin Remodeling
  • ISWI/NURF/CHRAC

Applications

Target

• FunctionCore histone-binding subunit that may target chromatin assembly factors, chromatin remodeling factors and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. Component of several complexes which regulate chromatin metabolism. These include the chromatin assembly factor 1 (CAF-1) complex, which is required for chromatin assembly following DNA replication and DNA repair; the core histone deacetylase (HDAC) complex, which promotes histone deacetylation and consequent transcriptional repression; the nucleosome remodeling and histone deacetylase complex (the NuRD complex), which promotes transcriptional repression by histone deacetylation and nucleosome remodeling; the PRC2/EED-EZH2 complex, which promotes repression of homeotic genes during development; and the NURF (nucleosome remodeling factor) complex.
• Sequence similaritiesBelongs to the WD repeat RBAP46/RBAP48/MSI1 family.
  Contains 6 WD repeats.
• Cellular localizationNucleus.

Target information above from: UniProt accession Q09028 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 395658 Chicken
  • Entrez Gene: 5928 Human
  • Entrez Gene: 19646 Mouse
  • Entrez Gene: 321726 Zebrafish
  • Omim: 602923 Human
  • SwissProt: Q9W7I5 Chicken
  • SwissProt: Q09028 Human
  • SwissProt: Q60972 Mouse

  • SwissProt: Q6P3H7 Zebrafish
  • Unigene: 16003 Human
  • Unigene: 12145 Mouse
  • Unigene: 76462 Zebrafish

  see all

• Alternative names
  CAF 1 subunit C antibodyCAF I 48 kDa subunit antibodyCAF I p48 antibody

  CAF-1 subunit C antibodyCAF-I 48 kDa subunit antibodyCAF-I p48 antibodyChromatin assembly factor 1 subunit C antibodyChromatin assembly factor I p48 subunit antibodyChromatin assembly factor/CAF 1 p48 subunit antibodyHistone binding protein RBBP4 antibodyHistone-binding protein RBBP4 antibodyMSI1 protein homolog antibodyNucleosome remodeling factor subunit RBAP48 antibodyNucleosome-remodeling factor subunit RBAP48 antibodyNURF 55 antibodyNURF55 antibodyRbAp 48 antibodyRBAP48 antibodyRBBP 4 antibodyRBBP-4 antibodyRBBP4 antibodyRBBP4_HUMAN antibodyRetinoblastoma binding protein 4 antibodyRetinoblastoma binding protein p48 antibodyRetinoblastoma-binding protein 4 antibodyRetinoblastoma-binding protein p48 antibody

  see all

Anti-RbAp48 antibody images

• 

  Immunocytochemistry/ Immunofluorescence - RbAp48 antibody (ab3536)

  ab3536 staining RbAp48 in human hella cells by immunocytochemistry/ Immunofluorescence.

• 

  Western blot - RbAp48 antibody (ab3536)
  
  
  Predicted band size : 47.5 kDa
  
  
  Western blot of RpAp48 on 1) Du-148 2) LNCaP and 3) Hep-G2 cell extracts using ab3536.