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Anti-RbAp48 antibody (ab3536)

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Overview

Product name

Anti-RbAp48 antibody
See all RbAp48 products (14) ...

Description

Rabbit polyclonal to RbAp48

Tested applications

WBmore details

Cross reactivity

Reacts with

Human

Predicted to work with

Mouse, Chicken, Xenopus laevis, Zebrafish

Immunogen

Synthetic peptide: MADKFAAFDDAVEEC, corresponding to amino acids 1-14 of Human RbAp 48.

MADKFAAFDD AVEEC

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

PBS, 1 mg/ml BSA, 0.05% sodium azide

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Primary antibody notes

Retinoblastoma protein (Rb) associated protein 48 (RbAp 48) is a nuclear protein that has been shown to interact directly with retinoblastoma protein and is a component of the mSin3 corepressor complex as are the histone deacetylase proteins (HDACs). RbAp 48 shares about 75% sequence identity with the closely related RbAp 46, and appears to be functionally related to the yeast MSI 1 protein in that overexpression of RbAp 48 suppresses heat shock sensitivity in a RAS pathway dependent manner. RbAp48 has also been demonstrated to be up-regulated by the Wilms’ tumor suppressor gene product, WT1. Additionally, cells transfected with RbAp 48 have shown strong inhibition of growth.

Clonality

Polyclonal

Isotype

IgG

  • Immunocytochemistry/ Immunofluorescence - RbAp48 antibody (ab3536)Immunocytochemistry/ Immunofluorescence - RbAp48 antibody (ab3536) image (enlarge)

  • Western blot - RbAp48 antibody (ab3536)Western blot - RbAp48 antibody (ab3536) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab3536 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

WB: Use at a concentration of 0.5 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 47.5 kDa). A larger ~60 kDa unknown protein from HeLa cell lysate is also detected. Can be blocked with RbAp 48 peptide

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Function

Core histone-binding subunit that may target chromatin assembly factors, chromatin remodeling factors and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. Component of several complexes which regulate chromatin metabolism. These include the chromatin assembly factor 1 (CAF-1) complex, which is required for chromatin assembly following DNA replication and DNA repair; the core histone deacetylase (HDAC) complex, which promotes histone deacetylation and consequent transcriptional repression; the nucleosome remodeling and histone deacetylase complex (the NuRD complex), which promotes transcriptional repression by histone deacetylation and nucleosome remodeling; the PRC2/EED-EZH2 complex, which promotes repression of homeotic genes during development; and the NURF (nucleosome remodeling factor) complex.

Sequence similarities

Belongs to the WD repeat RBAP46/RBAP48/MSI1 family.
Contains 6 WD repeats.

Cellular localization

Nucleus.

Target information above from: UniProt accessionQ09028 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • CAF 1 subunit C antibody
  • CAF I 48 kDa subunit antibody
  • CAF I p48 antibody
  • CAF-1 subunit C antibody
  • CAF-I 48 kDa subunit antibody
  • CAF-I p48 antibody
  • Chromatin assembly factor 1 subunit C antibody
  • Chromatin assembly factor I p48 subunit antibody
  • Chromatin assembly factor/CAF 1 p48 subunit antibody
  • Histone binding protein RBBP4 antibody
  • Histone-binding protein RBBP4 antibody
  • MSI1 protein homolog antibody
  • Nucleosome remodeling factor subunit RBAP48 antibody
  • Nucleosome-remodeling factor subunit RBAP48 antibody
  • NURF 55 antibody
  • NURF55 antibody
  • RbAp 48 antibody
  • RBAP48 antibody
  • RBBP 4 antibody
  • RBBP-4 antibody
  • RBBP4 antibody
  • RBBP4_HUMAN antibody
  • Retinoblastoma binding protein 4 antibody
  • Retinoblastoma binding protein p48 antibody
  • Retinoblastoma-binding protein 4 antibody
  • Retinoblastoma-binding protein p48 antibody
see all

Anti-RbAp48 antibody images:

  Immunocytochemistry/ Immunofluorescence - RbAp48 antibody (ab3536)

Immunocytochemistry/ Immunofluorescence - RbAp48 antibody (ab3536)

ab3536 staining RbAp48 in human hella cells by immunocytochemistry/ Immunofluorescence.

  Western blot - RbAp48 antibody (ab3536)

Western blot - RbAp48 antibody (ab3536)



Predicted band size : 47.5 kDa


Western blot of RpAp48 on 1) Du-148 2) LNCaP and 3) Hep-G2 cell extracts using ab3536.

References for Anti-RbAp48 antibody (ab3536)

This product has been referenced in:

  • Sridharan R & Smale ST Predominant interaction of both Ikaros and Helios with the NuRD complex in immature thymocytes. J Biol Chem 282:30227-38 (2007). WB; Human.Read more (PubMed: 17681952) »

See 1 publication for this product

Publishing research using ab3536? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"