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Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab3536 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB
  • Application notesWB: Use at a concentration of 0.5 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 47.5 kDa). A larger ~60 kDa unknown protein from HeLa cell lysate is also detected. Can be blocked with RbAp 48 peptide

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionCore histone-binding subunit that may target chromatin assembly factors, chromatin remodeling factors and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. Component of several complexes which regulate chromatin metabolism. These include the chromatin assembly factor 1 (CAF-1) complex, which is required for chromatin assembly following DNA replication and DNA repair; the core histone deacetylase (HDAC) complex, which promotes histone deacetylation and consequent transcriptional repression; the nucleosome remodeling and histone deacetylase complex (the NuRD complex), which promotes transcriptional repression by histone deacetylation and nucleosome remodeling; the PRC2/EED-EZH2 complex, which promotes repression of homeotic genes during development; and the NURF (nucleosome remodeling factor) complex.
    • Sequence similaritiesBelongs to the WD repeat RBAP46/RBAP48/MSI1 family.
      Contains 6 WD repeats.
    • Cellular localizationNucleus.
    • Target information above from: UniProt accession Q09028 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
    • Alternative names
        CAF 1 subunit C antibodyCAF I 48 kDa subunit antibodyCAF I p48 antibody
        CAF-1 subunit C antibodyCAF-I 48 kDa subunit antibodyCAF-I p48 antibodyChromatin assembly factor 1 subunit C antibodyChromatin assembly factor I p48 subunit antibodyChromatin assembly factor/CAF 1 p48 subunit antibodyHistone binding protein RBBP4 antibodyHistone-binding protein RBBP4 antibodyMSI1 protein homolog antibodyNucleosome remodeling factor subunit RBAP48 antibodyNucleosome-remodeling factor subunit RBAP48 antibodyNURF 55 antibodyNURF55 antibodyRbAp 48 antibodyRBAP48 antibodyRBBP 4 antibodyRBBP-4 antibodyRBBP4 antibodyRBBP4_HUMAN antibodyRetinoblastoma binding protein 4 antibodyRetinoblastoma binding protein p48 antibodyRetinoblastoma-binding protein 4 antibodyRetinoblastoma-binding protein p48 antibody
      see all

    Anti-RbAp48 antibody images

    • ab3536 staining RbAp48 in human hella cells by immunocytochemistry/ Immunofluorescence.



    • Predicted band size : 47.5 kDa


      Western blot of RpAp48 on 1) Du-148 2) LNCaP and 3) Hep-G2 cell extracts using ab3536.

    References for Anti-RbAp48 antibody (ab3536)

    This product has been referenced in:
    • Sridharan R & Smale ST Predominant interaction of both Ikaros and Helios with the NuRD complex in immature thymocytes. J Biol Chem 282:30227-38 (2007). WB ; Human . Read more (PubMed: 17681952) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"