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Overview

  • Product nameRbBP5 protein (Tagged-His Tag)See all RbBP5 proteins and peptides ...
  • Protein descriptionRecombinant fragment, corresponding to amino acids 359-538 (180 amino acids) of Human RbBP5 with an N terminal His tag. Predicted mwt: 20 kDa; UniProt ID: Q15291.
  • Expression hostE. coli

    • Properties

  • Purification notesPurity level >85%
    This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag.
  • FormLyophilised:Reconstitute with 148 µl distilled water.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
  • Concentration information loading...
  • Additional notesProtein Identity confirmed by Mass Spectrometry (MS/MS) (acquired on initial reference batch).
  • Sequence notesKSEPEQTGADAAEDEEVDVTSVDPIAAFCSSDEELEDSKA LLYLPIAPEVEDP EENPYGPPPDAVQTSLMDEGASSEK KRQSSADGSQPPKKKPKTTNIELQGVPN DEVHPLLGVK GDGKSKKKQAGRPKGSKGKEKDSPFKPKLYKGDRGLPLEG SAK GKVQAELSQPLTAGGAISELL
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab91953 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    MS
    SDS-PAGE
  • Application notesMS: Use at an assay dependent dilution.
    SDS-PAGE: Use at an assay dependent dilution.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        RBBP 5RBBP-5RBBP5
        RBBP5_HUMANRBQ 3RBQ3Retinoblastoma binding protein 5Retinoblastoma binding protein RBQ3Retinoblastoma binding protein5Retinoblastoma-binding protein 5Retinoblastoma-binding protein RBQ-3SWD1SWD1, Set1c WD40 repeat protein, homolog
      see all
  • FunctionAs part of the MLL1/MLL complex it is involved in methylation and dimethylation at 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation.
  • Tissue specificityUbiquitously expressed.
  • Sequence similaritiesContains 6 WD repeats.
  • Post-translational
    modifications    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationNucleus.

    • Target information above from: UniProt accession Q15291 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    RbBP5 protein (Tagged-His Tag) images

    • SDS-PAGE - RbBP5 protein (Tagged-His Tag) (ab91953)
      SDS-PAGE - RbBP5 protein (Tagged-His Tag) (ab91953)
      The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images coloured control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.

    References for RbBP5 protein (Tagged-His Tag) (ab91953)

    ab91953 has not yet been referenced specifically in any publications.

    Publishing research using ab91953 ? Please let us know so that we can cite the reference in this datasheet

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