Native E. coli beta Galactosidase protein (ab78141)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Suitable for: SDS-PAGE
Description
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Product name
Native E. coli beta Galactosidase protein
See all beta Galactosidase proteins and peptides -
Biological activity
Does not react with human serum. -
Purity
> 95 % SDS-PAGE. -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Native -
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Species
Escherichia coli -
Predicted molecular weight
114 kDa -
Amino acids
2 to 1024
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab78141 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
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Form
Liquid -
Additional notes
Does not react with human serum. -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Constituents: 48% Urea, 0.078% Beta mercaptoethanol, 0.316% Tris HCl
General Info
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Alternative names
- Beta D galactosidase
- Beta gal
- Beta galactosidase
see all -
Relevance
Beta galactosidase is coded by a gene (lac z) in the lac operon of Escherichia coli. It is a metalloenzyme that splits lactose into glucose and galactose. It hydrolyzes terminal, non-reducing beta-D-galactose residues in beta-D-galactosides. Activation by cations seems to be substrate dependent. K+, Na+, NH4+, Rb+, Cs+ and Mn++ all activate enzyme activity based upon the substrate used. -
Cellular localization
Cytoplasmic
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab78141 has been referenced in 1 publication.
- Ruggiero L et al. Diurnal, localized exposure of phosphatidylserine by rod outer segment tips in wild-type but not Itgb5-/- or Mfge8-/- mouse retina. Proc Natl Acad Sci U S A 109:8145-8 (2012). PubMed: 22566632