Recombinant Hepatitis C Virus NS3 + NS4A (mutated D168V) protein (ab198098)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Active: Yes
- Tags: His-DDDDK tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
Description
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Product name
Recombinant Hepatitis C Virus NS3 + NS4A (mutated D168V) protein
See all Hepatitis C Virus NS3 + NS4A proteins and peptides -
Biological activity
Specific Activity: 45 pmole/min/µg.
Assay Conditions: Assay was performed in 100 µl HCV assay buffer containing 50 mM Tris, pH7.4, 150 mM NaCl, 5 mM DTT, 10% glycerol, and 5 µM fluorescently labeled HCV substrate peptide. Reaction was continuously monitored at room temperature for 20 min. at ex350/em500.
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Purity
> 90 % SDS-PAGE. -
Expression system
Escherichia coli -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Sequence
MDYKDDDDKHHHHHHGSVVIVGRIILSGSGSITAYSQQTRGVLGCIITSL TGRDKNQVEGEVQVVSTATQSFLATCINGVCWTVYHGAGSKTLAGPKGPI TQMYTNVDLDLVGWQAPPGARSMTPCSCGSSDLYLVTRHADVIPVRRRGD SRGSLLSPRPVSYLKGSSGGPLLCPSGHVVGVFRAAVCTRGVAKAVVFIP VESMETTMRS -
Predicted molecular weight
22 kDa including tags -
Modifications
mutated D168V -
Tags
His-DDDDK tag N-Terminus -
Additional sequence information
Fusion protein of aa21-32 of NS4A, linker (4 aa) and aa3-181 of NS3 with Asp-to-Val mutation on aa168.
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab198098 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Functional Studies
SDS-PAGE
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Form
Liquid -
Additional notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
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Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.63% Tris HCl, 2.32% Sodium chloride, 0.2% Triton X-100, 0.08% Beta mercaptoethanol, 30% Glycerol (glycerin, glycerine)
Contains 100 µg/ml DDDDK peptide.This product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab198098 has not yet been referenced specifically in any publications.