Recombinant Human 14-3-3 Theta + Tau protein (ab116444)
Key features and details
- Expression system: Escherichia coli
- Purity: > 80% Purified via His tag
- Tags: His tag N-Terminus
- Suitable for: MS, SDS-PAGE
Description
-
Product name
Recombinant Human 14-3-3 Theta + Tau protein
See all 14-3-3 Theta + Tau proteins and peptides -
Purity
> 80 % Purified via His tag.
ab116444 was purified via Ni2+-NTA agarose columns. -
Expression system
Escherichia coli -
Accession
-
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
-
Species
Human -
Sequence
MGSSHHHHHHSSGLVPRGSHMASMTGGQQMGRGSMEKTELIQKAKLAEQA ERYDDMATCMKAVTEQGAELSNEERNLLSVAYKNVVGGRRSAWRVISSIE QKTDTSDKKLQLIKDYREKVESELRSICTTVLELLDKYLIANATNPESKV FYLKMKGDYFRYLAEVACGDDRKQTIDNSQGAYQEAFDISKKEMQPTHPI RLGLALNFSVFYYEILNNPELACTLAKTAFDEAIAELDTLNEDSYKDSTL IMQLLRDNLTLWTSDSAGEECDAAEGAEN -
Predicted molecular weight
31 kDa including tags -
Amino acids
1 to 245 -
Tags
His tag N-Terminus
-
Associated products
-
Related Products
Specifications
Our Abpromise guarantee covers the use of ab116444 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
Applications
Mass Spectrometry
SDS-PAGE
-
Mass spectrometry
MALDI-TOF-TOF -
Form
Liquid -
Additional notes
ab116444 was alkylated, digested with trypsin and the mass of the resultant peptides determined by MADLI-TOF/TOF mass spectrometry. The peptides obtained gave 64 % sequence coverage of 14-3-3 tau + theta. Theoretical pI: 5.2
-
Concentration information loading...
Preparation and Storage
-
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.012% Benzamidine, 0.004% PMSF, 0.02% DTT, 0.6% HEPES, 50% Glycerol
General Info
-
Alternative names
- 14-3-3 protein T cell
- 14-3-3 protein T-cell
- 14-3-3 protein tau
see all -
Function
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. -
Tissue specificity
Abundantly expressed in brain, heart and pancreas, and at lower levels in kidney and placenta. Up-regulated in the lumbar spinal cord from patients with sporadic amyotrophic lateral sclerosis (ALS) compared with controls, with highest levels of expression in individuals with predominant lower motor neuron involvement. -
Sequence similarities
Belongs to the 14-3-3 family. -
Post-translational
modificationsSer-232 is probably phosphorylated by CK1. -
Cellular localization
Cytoplasm. In neurons, axonally transported to the nerve terminals. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab116444 has not yet been referenced specifically in any publications.