Recombinant Human 14-3-3 eta/YWHAH protein (ab116442)
Key features and details
- Expression system: Escherichia coli
- Purity: > 80% Purified via His tag
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE, MS
Description
-
Product name
Recombinant Human 14-3-3 eta/YWHAH protein
See all 14-3-3 eta/YWHAH proteins and peptides -
Purity
> 80 % Purified via His tag.
ab116442 was purified by Ni2+-NTA agarose -
Expression system
Escherichia coli -
Accession
-
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
-
Species
Human -
Sequence
MGSSHHHHHHSSGLVPRGSHMASMTGGQQMGRGSMGDREQLLQRARLAEQ AERYDDMASAMKAVTELNEPLSNEDRNLLSVAYKNVVGARRSSWRVISSI EQKTMADGNEKKLEKVKAYREKIEKELETVCNDVLSLLDKFLIKNCNDFQ YESKVFYLKMKGDYYRYLAEVASGEKKNSVVEASEAAYKEAFEISKEQMQ PTHPIRLGLALNFSVFYYEIQNAPEQACLLAKQAFDDAIAELDTLNEDSY KDSTLIMQLLRDNLTLWTSDQQDEEAGEGN -
Predicted molecular weight
32 kDa including tags -
Amino acids
1 to 246 -
Tags
His tag N-Terminus
-
Specifications
Our Abpromise guarantee covers the use of ab116442 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
Applications
SDS-PAGE
Mass Spectrometry
-
Mass spectrometry
MALDI-TOF-TOF -
Form
Liquid -
Additional notes
ab116442 was alkylated, digested with trypsin and the mass of the resultant peptides determined by MADLI-TOF/TOF. The peptides obtained gave 67% sequence coverage of 14-3-3 eta/YWHAH. Theoretical pI: 5.3
This product was previously labelled as 14-3-3 eta
-
Concentration information loading...
Preparation and Storage
-
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.012% Benzamidine, 0.003% PMSF, 0.02% DTT, 0.6% HEPES, 50% Glycerol
General Info
-
Alternative names
- 14 3 3 protein eta
- 14-3-3 eta
- 14-3-3 protein eta
see all -
Function
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. -
Tissue specificity
Expressed mainly in the brain and present in other tissues albeit at lower levels. -
Sequence similarities
Belongs to the 14-3-3 family. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab116442 has not yet been referenced specifically in any publications.