Recombinant Human APAF1 protein (Tagged) (ab198064)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 90% SDS-PAGE
- Tags: His-DDDDK tag N-Terminus
- Suitable for: SDS-PAGE
Description
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Product name
Recombinant Human APAF1 protein (Tagged) -
Purity
> 90 % SDS-PAGE.
Affinity purified. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MHHHHHHDYKDDDDKDAKARNCLLQHREALEKDIKTSYIMDHMISDGFLT ISEEEKVRNEPTQQQRAAMLIKMILKKDNDSYVSFYNALLHEGYKDLAAL LHDGIPVVSSSSVRTVLCEGGVPQRPVVFVTRKKLVNAIQQKLSKLKGEP GWVTIHGMAGCGKSVLAAEAVRDHSLLEGCFPGGVHWVSVGKQDKSGLLM KLQNLCTRLDQDESFSQRLPLNIEEAKDRLRILMLRKHPRSLLILDDVWD SWVLKAFDSQCQILLTTRDKSVTDSVMGPKYVVPVESSLGKEKGLEILSL FVNMKKADLPEQAHSIIKECKGSPLVVSLIGALLRDFPNRWEYYLKQLQN KQFKRIRKSSSYDYEALDEAMSISVEMLREDIKDYYTDLSILQKDVKVPT KVLCILWDMETEEVEDILQEFVNKSLLFCDRNGKSFRYYLHDLQVDFLTE KNCSQLQDLHKKIITQFQRYHQPHTLSPDQEDCMYWYNFLAYHMASAKMH KELCALMFSLDWIKAKTELVGPAHLIHEFVEYRHILDEKDCAVSENFQEF LSLNGHLLGRQPFPNIVQLGLCEPETSEVYQQAKLQAKQEVDNGMLYLEW INKKNITNLSRLVVRPHTDAVYHACFSEDGQRIASCGADKTLQVFKAETG EKLLEIKAHEDEVLCCAFSTDDRFIATCSVDKKVKIWNSMTGELVHTYDE HSETQVNCCHFTNSSHHLLLATGSSDCFLKLWDLNQKECRNTMFGHTNSV NHCRFSPDDKLLASCSADGTLKLWDATSANERKSINVKQFFLNLEDPQED MEVIVKCCSWSADGARIMVAAKNKIFLFDIHTSGLLGEIHTGHHSTIQYC DFSPQNHLAVVALSQYCVELWNTDSRSKVADCRGHLSWVHGVMFSPDGSS FLTSSDDQTIRLWETKKVCKNSAVMLKQEVDVVFQENEVMVLAVDHIRRL QLINGRTGQIDYLTEAQVSCCCLSPHLQYIAFGDENGAIEILELVNNRIF QSRFQHKKTVWHIQFTADEKTLISSSDDAEIQVWNWQLDKCIFLRGHQET VKDFRLLKNSRLLSWSFDGTVKVWNIITGNKEKDFVCHQGTVLSCDISHD ATKFSSTSADKTAKIWSFDLLLPLHELRGHNGCVRCSAFSVDSTLLATGD DNGEIRIWNVSNGELLHLCAPLSEEGAATHGGWVTDLCFSPDGKMLISAG GYIKWWNVVTGESSQTFYTNGTNLKKIHVSPDFKTYVTVDNLGILYILQT LE -
Predicted molecular weight
143 kDa including tags -
Amino acids
2 to 1237 -
Tags
His-DDDDK tag N-Terminus -
Additional sequence information
This product is for the mature full length Isoform 2 protein from aa 2 to 1237. The initial Met is not included. GenBank Accession No. NM_013229.
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab198064 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 0.04% Tween, 20% Glycerol (glycerin, glycerine)
80 µg/ml DDDDK peptide
General Info
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Alternative names
- APAF 1
- Apaf-1
- APAF_HUMAN
see all -
Function
Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis. -
Tissue specificity
Ubiquitous. Highest levels of expression in adult spleen and peripheral blood leukocytes, and in fetal brain, kidney and lung. Isoform 1 is expressed in heart, kidney and liver. -
Sequence similarities
Contains 1 CARD domain.
Contains 1 NB-ARC domain.
Contains 13 WD repeats. -
Domain
The CARD domain mediates interaction with APIP. -
Cellular localization
Cytoplasm. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab198064 has been referenced in 1 publication.
- Wang Q et al. APAF1-Binding Long Noncoding RNA Promotes Tumor Growth and Multidrug Resistance in Gastric Cancer by Blocking Apoptosome Assembly. Adv Sci (Weinh) 9:e2201889 (2022). PubMed: 35975461