Recombinant Human Annexin V/ANXA5 protein (His tag) (ab157342)
Key features and details
- Expression system: Escherichia coli
- Purity: > 98% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Tags: His tag C-Terminus
- Suitable for: SDS-PAGE
Description
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Product name
Recombinant Human Annexin V/ANXA5 protein (His tag)
See all Annexin V/ANXA5 proteins and peptides -
Purity
> 98 % SDS-PAGE.
ab157342 is lyophilized from 0.22 µm filtered solution. -
Endotoxin level
< 1.000 Eu/µg -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MAQVLRGTVTDFPGFDERADAETLRKAMKGLGTDEESILTLLTSRSNAQR QEISAAFKTLFGRDLLDDLKSELTGKFEKLIVALMKPSRLYDAYELKHAL KGAGTNEKVLTEIIASRTPEELRAIKQVYEEEYGSSLEDDVVGDTSGYYQ RMLVVLLQANRDPDAGIDEAQVEQDAQALFQAGELKWGTDEEKFITIFGT RSVSHLRKVFDKYMTISGFQIEETIDRETSGNLEQLLLAVVKSIRSIPAY LAETLYYAMKGAGTDDHTLIRVMVSRSEIDLFNIRKEFRKNFATSLYSMI KGDTSGDYKKALLLLCGEDD -
Predicted molecular weight
37 kDa including tags -
Amino acids
1 to 320 -
Tags
His tag C-Terminus
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Associated products
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Related Products
- Anti-Annexin V/ANXA5 antibody [EPR3980] (ab108194)
- Anti-Annexin V/ANXA5 antibody [EPR3979] (ab108321)
- Anti-Annexin V/ANXA5 antibody (ab137823)
- Anti-Annexin V/ANXA5 antibody (ab14196)
- Anti-6X His tag® antibody [HIS.H8] (ab18184)
- Anti-6X His tag® antibody [4D11] (ab5000)
- Anti-Annexin V/ANXA5 antibody [1F4-1A5] (ab54775)
- Anti-6X His tag® antibody (ab9108)
Specifications
Our Abpromise guarantee covers the use of ab157342 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
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Form
Lyophilized -
Additional notes
This product was previously labelled as Annexin V.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Constituent: PBS
5-10% trehalose is commonly used for freeze drying, and after reconstitution, the trehalose is mostly about 3-5%. -
ReconstitutionReconstitute with sterile deionized water to a concentration of 400 µg/ml.
General Info
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Alternative names
- Anchorin CII
- Annexin 5
- Annexin A5
see all -
Function
This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade. -
Involvement in disease
Pregnancy loss, recurrent, 3 -
Sequence similarities
Belongs to the annexin family.
Contains 4 annexin repeats. -
Domain
The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.
A pair of annexin repeats may form one binding site for calcium and phospholipid. -
Post-translational
modificationsS-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex. - Information by UniProt
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab157342 has not yet been referenced specifically in any publications.