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Question (73135) | Recombinant Human CCN1 protein (ab50074)

functional studies with this protein on human lung fibroblast cells
obtained inconsistent results
dissolved protein in water 9100 ul)
20 ul aliquots stored at -20 degree

WB: detects Bcl2 and p16
titration from 5 ng to 2 ug, 250 ng 24-48h --> gave good results the first time (fresh prep)
next aliquot gave no upregulation in WB and ICC
protein seems to loose activity when stored in aliquots at -20 degree

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