Recombinant Human HAO1/GOX protein (ab113144)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Tags: His tag N-Terminus, DDDDK tag N-Terminus
- Suitable for: SDS-PAGE
Description
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Product name
Recombinant Human HAO1/GOX protein
See all HAO1/GOX proteins and peptides -
Purity
> 95 % SDS-PAGE.
ab113144 was purified using conventional chromatography. -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MRGSHHHHHHGMASMTGGQQMGRDLYDDDDKDRWGSMLPRLICINDYEQH AKSVLPKSIYDYYRSGAN DEETLADNIAAFSRWKLYPRMLRNVAETDL STSVLGQRVSMPICVGATAMQRMAHVDGELATVRACQSLGTGMML SSW ATSSIEEVAEAGPEALRWLQLYIYKDREVTKKLVRQAEKMGYKAIFVTVD TPYLGNRLDDVRNRFKLPPQLR MKNFETSTLSFSPEENFGDDSGLAAY VAKAIDPSISWEDIKWLRRLTSLPIVAKGILRGDDAREAVKHGLNGILV SNHGARQLDGVPATIDVLPEIVEAVEGKVEVFLDGGVRKGTDVLKALAL GAKAVFVGRPIVWGLAFQGEKGVQDV LEILKEEFRLAMALSGCQNVKV IDKTLVRKNPLAVSKI -
Predicted molecular weight
45 kDa including tags -
Amino acids
1 to 370 -
Tags
His tag N-Terminus , DDDDK tag N-Terminus
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab113144 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
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Mass spectrometry
MALDI-TOF -
Form
Liquid -
Additional notes
Protein previously labeled as HAO1.
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.32% Tris HCl, 20% Glycerol (glycerin, glycerine), 2.92% Sodium chloride
General Info
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Alternative names
- (S) 2 hydroxy acid oxidase
- Glycolate oxidase
- GOX
see all -
Function
Has 2-hydroxyacid oxidase activity. Most active on the 2-carbon substrate glycolate, but is also active on 2-hydroxy fatty acids, with high activity towards 2-hydroxy palmitate and 2-hydroxy octanoate. -
Tissue specificity
Liver. -
Pathway
Organic acid metabolism; glycolate degradation; 3-phospho-D-glycerate from glycolate: step 1/4. -
Sequence similarities
Belongs to the FMN-dependent alpha-hydroxy acid dehydrogenase family.
Contains 1 FMN hydroxy acid dehydrogenase domain. -
Cellular localization
Peroxisome. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab113144 has been referenced in 1 publication.
- Wang M et al. High throughput cell-based assay for identification of glycolate oxidase inhibitors as a potential treatment for Primary Hyperoxaluria Type 1. Sci Rep 6:34060 (2016). PubMed: 27670739