Recombinant Human PHLPP2 protein (ab125556)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% Densitometry
- Suitable for: WB, SDS-PAGE
Description
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Product name
Recombinant Human PHLPP2 protein -
Purity
> 95 % Densitometry.
ab125556 is >95% pure. -
Expression system
Escherichia coli -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Predicted molecular weight
56 kDa including tags -
Amino acids
766 to 1043
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Specifications
Our Abpromise guarantee covers the use of ab125556 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Western blot
SDS-PAGE
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 25% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.003% EDTA, 0.79% Tris HCl, 0.004% DTT, 0.002% PMSF, 0.31% Glutathione
General Info
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Alternative names
- KIAA0931
- PH domain and leucine rich repeat protein phosphatase 2
- PH domain leucine rich repeat protein phosphatase like
see all -
Function
Protein phosphatase that mediates dephosphorylation of 'Ser-473' of AKT1, 'Ser-660' of PRKCB isoform beta-II and 'Ser-657' of PRKCA. AKT1 regulates the balance between cell survival and apoptosis through a cascade that primarily alters the function of transcription factors that regulate pro- and antiapoptotic genes. Dephosphorylation of 'Ser-473' of AKT1 triggers apoptosis and decreases cell proliferation. Also controls the phosphorylation of AKT3. Dephosphorylation of PRKCA and PRKCB leads to their destabilization and degradation. Inhibits cancer cell proliferation and may act as a tumor suppressor. -
Tissue specificity
In colorectal cancer tissue, expression is highest in the surface epithelium of normal colonic mucosa adjacent to the cancer tissue but is largely excluded from the crypt bases. Expression is lost or significantly decreased in 80% of tested tumors (at protein level). -
Sequence similarities
Contains 22 LRR (leucine-rich) repeats.
Contains 1 PH domain.
Contains 1 PP2C-like domain. -
Cellular localization
Cytoplasm. Membrane. Nucleus. In colorectal cancer tissue, expression is concentrated in the cytoplasm and nucleus. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab125556 has not yet been referenced specifically in any publications.