Recombinant Human TATA binding protein TBP (ab81897)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Tags: His tag N-Terminus
- Suitable for: GSA, SDS-PAGE, WB
Description
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Product name
Recombinant Human TATA binding protein TBP
See all TATA binding protein TBP proteins and peptides -
Biological activity
1 unit equals 1 nanogram of purified protein. -
Purity
> 95 % SDS-PAGE. -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MDQNNSLPPY AQGLASPQGA MTPGIPIFSP MMPYGTGLTP QPIQ NTNSL SILEEQQRQQ QQQQQQQQQQ QQQQQQQQQQ QQQQQQQQQQ QQQQQQAVAA AAV QQSTSQ QATQGTSGQA PQLFHSQTLT TAPLPGTTPL YPSPMTPMTP ITPATPASES SG IVPQLQN IVSTVNLGCK LDLKTIALRA RNAEYNPKRF AAVIMRIREP RTTALIFSSG K MVCTGAKS EEQSRLAARK YARVVQKLGF PAKFLDFKIQ NMVGSCDVKF PIRLEGLVLT HQQFSSYEP ELFPGLIYRM IKPRIVLLIF VSGKVVLTGA KVRAEIYEAF ENIYPILKG FRKTT -
Predicted molecular weight
39 kDa -
Amino acids
1 to 339 -
Tags
His tag N-Terminus
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab81897 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Gel Supershift Assays
SDS-PAGE
Western blot
EMSA
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Form
Liquid -
Additional notes
1 unit equals 1 nanogram of purified protein. -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.9
Constituents: 0.75% Potassium chloride, 0.0154% DTT, 0.316% Tris HCl, 0.00584% EDTA, 20% Glycerol (glycerin, glycerine)
General Info
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Alternative names
- GTF2D
- GTF2D1
- HDL4
see all -
Function
General transcription factor that functions at the core of the DNA-binding multiprotein factor TFIID. Binding of TFIID to the TATA box is the initial transcriptional step of the pre-initiation complex (PIC), playing a role in the activation of eukaryotic genes transcribed by RNA polymerase II. Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC (preinitiation complex) during RNA polymerase I-dependent transcription. The rate of PIC formation probably is primarily dependent on the rate of association of SL1 with the rDNA promoter. SL1 is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA. -
Tissue specificity
Widely expressed, with levels highest in the testis and ovary. -
Involvement in disease
Defects in TBP are the cause of spinocerebellar ataxia type 17 (SCA17) [MIM:607136]. Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCA17 is an autosomal dominant cerebellar ataxia (ADCA) characterized by widespread cerebral and cerebellar atrophy, dementia and extrapyramidal signs. The molecular defect in SCA17 is the expansion of a CAG repeat in the coding region of TBP. Longer expansions result in earlier onset and more severe clinical manifestations of the disease. -
Sequence similarities
Belongs to the TBP family. -
Cellular localization
Nucleus. - Information by UniProt
Images
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All lanes : Anti-TATA binding protein TBP antibody - Nuclear Loading Control and ChIP Grade (ab63766) at 1 µg/ml
Lane 1 :Recombinant Human TATA binding protein TBP (ab81897) at 0.1 µg
Lane 2 :Recombinant Human TATA binding protein TBP (ab81897) at 0.01 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 10 seconds
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab81897 has been referenced in 1 publication.
- Grabherr MG et al. Exploiting nucleotide composition to engineer promoters. PLoS One 6:e20136 (2011). PubMed: 21625601