Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Molecular weight
      115 kDa including tags
    • Amino acids
      247 to 646

Specifications

Our Abpromise guarantee covers the use of ab125596 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    SDS-PAGE

  • Purity
    > 75 % SDS-PAGE.
    Assessed by densitometry.
  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.31% Glutathione, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol, 0.29% Sodium chloride

General Info

  • Alternative names
    • DKFZp686I18166
    • EC 2.7.10.2
    • FLJ16446
    • MGC105683
    • OTTHUMP00000231338
    • OTTHUMP00000231339
    • Wee 1
    • WEE 1 homolog 1 (S. pombe)
    • WEE1
    • Wee1 homolog
    • WEE1 homolog (S. pombe)
    • WEE1 homolog S. pombe
    • Wee1 like protein kinase
    • Wee1 tyrosine kinase
    • Wee1+ homolog
    • Wee1+ S. pombe homolog
    • WEE1, S. pombe, homolog of
    • WEE1, somatic
    • Wee1-like protein kinase
    • WEE1_HUMAN
    • WEE1A
    • Wee1A kinase
    • WEE1hu
    see all
  • Function
    Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation.
  • Sequence similarities
    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WEE1 subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Phosphorylated during M and G1 phases. Also autophosphorylated.
    Ubiquitinated and degraded at the onset of G2/M phase.
  • Cellular localization
    Nucleus.
  • Information by UniProt

Images

References

ab125596 has not yet been referenced specifically in any publications.

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