Recombinant Protein A + Protein G (ab52213)
Key features and details
- Expression system: Escherichia coli
- Purity: > 98% SDS-PAGE
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE
Description
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Product name
Recombinant Protein A + Protein G -
Biological activity
Protein A+G binds to all IgG subclasses (from various mammalian species). -
Purity
> 98 % SDS-PAGE. -
Expression system
Escherichia coli -
Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Tags
His tag N-Terminus -
Additional sequence information
The molecular weight including the His tag is 59.7 kDa Protein A: P02976 Protein G: P19909
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Associated products
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Positive Controls
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab52213 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Affinity Purification
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Form
Lyophilized -
Additional notes
This product is manufactured by BioVision, an Abcam company and was previously called 6502 Protein A/G. 6502-1 is the same size as the 1 mg size of ab52213.
This product is a genetically engineered fusion protein that combines the IgG binding profiles of both Protein A and Protein G. Protein A/G contains 6x His-tag on the N-terminus, five Ig-binding regions of protein A fusion with three Ig-binding region of protein G. The 6x His-tag on the N-terminus can be used for affinity purification or for protein A/G detection using an anti-His-tag antibody. Non-specific binding regions, including cell wall binding regions, albumin binding regions and other non-specific binding regions have been eliminated from this fusion protein A/G, to ensure the maximum specific IgG binding.
Protein A+G binds to all IgG subclasses (from various mammalian species).
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Constituent: 0.87% Sodium chloride
Without Mg+2 and Ca+2 -
ReconstitutionReconstitute in water to a concentration of 5 mg/ml.
General Info
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Cellular localization
Protein A: Secreted; cell wall. Protein G: Cell Wall and Secreted
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab52213 has been referenced in 1 publication.
- Ettrup KS et al. The anatomy of the Göttingen minipig hypothalamus. J Chem Neuroanat 39:151-65 (2010). PubMed: 20043984