Recombinant human Hsp70 protein (ab80031)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 90% SDS-PAGE
- Active: Yes
- Suitable for: WB, ELISA, Functional Studies, SDS-PAGE
Description
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Product name
Recombinant human Hsp70 protein
See all Hsp70 proteins and peptides -
Biological activity
ab80031 tested positive for ATPase activity using a Malachite Green assay.
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Purity
> 90 % SDS-PAGE.
ab80031 is Multi-step chromatographically purified. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Amino Acid Sequence 1
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Species
Human -
Sequence
MAKAAAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERL IGDAAKNQVALNPQNTVFDAKRLIGRKFGDPVVQSDMKHWPFQVINDGDK PKVQVSYKGETKAFYPEEISSMVLTKMKEIAEAYLGYPVTNAVITVPAYF NDSQRQATKDAGVIAGLNVLRIINEPTAAAIAYGLDRTGKGERNVLIFDL GGGTFDVSILTIDDGIFEVKATAGDTHLGGEDFDNRLVNHFVEEFKRKHK KDISQNKRAVRRLRTACERAKRTLSSSTGASLEIDSLFEGIDFYTSITRA RFEELCSDLFRSTLEPVEKALRDAKLDKAQIHDLVLVGGSTRIPKVQKLL QDFFNGRDLNKSINPDEAVGYGAAVQAAILMGDKSENVQDLLLLDVAPLS LGLETAGGVMTALIKRNSTIPTKQTQIFTTYSDNQPGVLIQVYEGERAMT KDNNLLGRFELSCIPPAPGVPQIEVTFDIDANGILNVTATKDSTGKANKI TITNDKGRLSKEEIERMVQEAEKYKAEDEVQRERVSAKNALESYAFNMKS AVEDEGLKGKISEADKKKVLDKCQEVISWLDANTLAEKDEFEHKRKELEQ VCNPIISGLYQGAGGPGPGGFGAQGPKGGSGSGPTIEEVD
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Amino Acid Sequence 2
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Species
Human -
Predicted molecular weight
70 kDa
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab80031 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Western blot
ELISA
Functional Studies
SDS-PAGE
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Form
Liquid -
Additional notes
ab80031 is endotoxin free. -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Constituents: 0.79% Tris HCl, 0.00292% EDTA, 10% Glycerol (glycerin, glycerine), 1.74% Sodium chloride
This product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- DnaK type molecular chaperone HSP70 1
- Epididymis secretory protein Li 103
- FLJ54303
see all -
Relevance
Function: In cooperation with other chaperones, the Hsp70 family stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. In case of rotavirus A infection, serves as a post-attachment receptor for the virus to facilitate entry into the cell. Tissue specificity: HSPA1B is testis-specific. -
Cellular localization
Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab80031 has not yet been referenced specifically in any publications.