Recombinant human NEK8 protein (ab204141)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 75% SDS-PAGE
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
Description
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Product name
Recombinant human NEK8 protein -
Biological activity
The specific activity of ab204141 was determined to be 5 nmol /min/mg as per activity assay protocol.
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Purity
> 75 % SDS-PAGE.
Assessed by densitometry. Affinity purified. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MEKYERIRVVGRGAFGIVHLCLRKADQKLVIIKQIPVEQMTKEERQAAQN ECQVLKLLNHPNVIEYYENFLEDKALMIAMEYAPGGTLAEFIQKRCNSLL EEETILHFFVQILLALHHVHTHLILHRDLKTQNILLDKHRMVVKIGDFGI SKILSSKSKAYTVVGTPCYISPELCEGKPYNQKSDIWALGCVLYELASLK RAFEAANLPALVLKIMSGTFAPISDRYSPELRQLVLSLLSLEPAQRPPLS HIMAQPLCIRALLNLHTDVGSVRMRRAEKSVAPSNTGSRTTSVRCRGIPR GPVRPAIPPPLSSVYAWGGGLG -
Predicted molecular weight
66 kDa including tags -
Amino acids
1 to 322 -
Tags
GST tag N-Terminus
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Associated products
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Related Products
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Substrate reagent
Specifications
Our Abpromise guarantee covers the use of ab204141 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Functional Studies
SDS-PAGE
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Form
Liquid -
Additional notes
ab64311 (Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
pH: 7.50
Constituents: 0.79% Tris HCl, 0.87% Sodium chloride, 0.31% Glutathione, 0.003% EDTA, 0.004% DTT, 0.002% PMSF, 25% Glycerol (glycerin, glycerine)This product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- JCK
- NEK 8
- NEK12A
see all -
Function
Required for renal tubular integrity. -
Tissue specificity
Highest expression in thyroid, adrenal gland and skin. Low levels in spleen, colon and uterus. Overexpressed in breast tumors, with highest expression in infiltrating ductal carcinomas and moderate levels in mucinous adenocarcinoma. -
Sequence similarities
Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.
Contains 1 protein kinase domain.
Contains 5 RCC1 repeats. -
Cellular localization
Cytoplasm. Predominantly cytoplasmic. - Information by UniProt
Images
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The specific activity of NEK8 (ab204141) was determined to be 1.8 nmol/min/mg as per activity assay protocol and was equivalent to 5 nmol/min/mg as per radiometric assay
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SDS PAGE analysis of ab204141
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SDS PAGE analysis of ab204141
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Sample Kinase Activity Plot showing ab204141. The specific activity of NEK8 was determined to be 5 nmol /min/mg as per activity assay protocol.
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SDS-PAGE showing ab204141.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab204141 has not yet been referenced specifically in any publications.